Thermosensitive hydrogel made of ferulic acid-gelatin and chitosan glycerophosphate

Yung Hsin Cheng, Shu Hua Yang, Chia Ching Liu, Amit Gefen, Feng Huei Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Reactive oxygen species-induced oxidative stress is involved in apoptosis of nucleus pulposus (NP) cells that can alter cellular phenotype and accelerate disc degeneration. Ferulic acid (FA) possesses an excellent antioxidant and anti-inflammatory properties. In the study, we developed the thermosensitive FA-gelatin/chitosan/glycerol phosphate (FA-G/C/GP) hydrogel which was applied as a sustained release system of FA to treat NP cells from the damage caused by oxidative stress. The gelation temperature of the FA-G/C/GP hydrogel was 32.17 °C. NP cells submitted to oxidative stress promoted by H2O 2, and post-treated with FA-G/C/GP exhibited down-regulation of MMP-3 and up-regulation aggrecan and type II collagen in mRNA level. The sulfated-glycosaminoglycan production was increased and the apoptosis was inhibited in the post-treatment group. The results suggest that the thermosensitive FA-G/C/GP hydrogel can treat NP cells from the damage caused by oxidative stress and may apply in minimally invasive surgery for NP regeneration.

Original languageEnglish
Pages (from-to)1512-1519
Number of pages8
JournalCarbohydrate Polymers
Issue number2
StatePublished - 15 Feb 2013


  • Ferulic acid
  • Nucleus pulposus
  • Oxidative stress
  • Thermosensitive hydrogel


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