TY - JOUR
T1 - The zebrafish as a model system for forebrain GnRH neuronal development
AU - Abraham, Eytan
AU - Palevitch, Ori
AU - Gothilf, Yoav
AU - Zohar, Yonathan
N1 - Funding Information:
We thank John Stubblefield for editing this manuscript. We also thank Kathy Kight, Dr. Ulrike Klenke and Dr. Susan Wray for their guidance and assistance. This study was supported by the US–Israel Bi-national Agricultural Research and Development (BARD) Foundation (Grant 3428-03) and by grants to Y.Z. from Maryland Sea Grant (SA7528051-M) and the National Science Foundation (IOB-0548620). This publication is Contribution No. 08-196 from the University of Maryland Centre Of Marine Biotechnology.
PY - 2009
Y1 - 2009
N2 - Development and function of the forebrain gonadotropin-releasing hormone (GnRH) neuronal system has long been the focus of study in various vertebrate species. This system is crucial for reproduction and an important model for studying tangential neuronal migration. In addition, the finding that multiple forms of GnRH exist in the CNS as well as in non-CNS tissues, coupled with the fact that GnRH fibers project to many CNS regions, implies that GnRH has a variety of functions in addition to its classic reproductive role. The study of the GnRH system and its functions is, however, limited by available model systems and methodologies. The transgenic (Tg) GnRH3:EGFP zebrafish line, in which GnRH3 neurons express EGFP, allows in vivo study of the GnRH3 system in the context of the entire animal. Coupling the use of this line with the attributes and molecular tools available in zebrafish has expanded our ability to study the forebrain GnRH system. Herein, we discuss the use of the Tg(GnRH3:EGFP) zebrafish line as a model for studying forebrain GnRH neurons, both in developing larvae and in sexually mature animals. We also discuss the potential use of this line to study regulation of GnRH3 system development.
AB - Development and function of the forebrain gonadotropin-releasing hormone (GnRH) neuronal system has long been the focus of study in various vertebrate species. This system is crucial for reproduction and an important model for studying tangential neuronal migration. In addition, the finding that multiple forms of GnRH exist in the CNS as well as in non-CNS tissues, coupled with the fact that GnRH fibers project to many CNS regions, implies that GnRH has a variety of functions in addition to its classic reproductive role. The study of the GnRH system and its functions is, however, limited by available model systems and methodologies. The transgenic (Tg) GnRH3:EGFP zebrafish line, in which GnRH3 neurons express EGFP, allows in vivo study of the GnRH3 system in the context of the entire animal. Coupling the use of this line with the attributes and molecular tools available in zebrafish has expanded our ability to study the forebrain GnRH system. Herein, we discuss the use of the Tg(GnRH3:EGFP) zebrafish line as a model for studying forebrain GnRH neurons, both in developing larvae and in sexually mature animals. We also discuss the potential use of this line to study regulation of GnRH3 system development.
KW - CNS
KW - Development
KW - GnRH
KW - Transgenic
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=70350015208&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2009.01.012
DO - 10.1016/j.ygcen.2009.01.012
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C2 - 19523393
AN - SCOPUS:70350015208
SN - 0016-6480
VL - 164
SP - 151
EP - 160
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2-3
ER -