The yeast VPS genes affect telomere length regulation

Ofer Rog; Sarit Smolikov; Anat Krauskopf; Martin Kupiec

doi:10.1007/s00294-004-0548-y

The yeast VPS genes affect telomere length regulation

Ofer Rog, Sarit Smolikov, Anat Krauskopf, Martin Kupiec^*

^*Corresponding author for this work

Biotechnology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Eukaryotic cells invest a large proportion of their genome in maintaining telomere length homeostasis. Among the 173 non-essential yeast genes found to affect telomere length, a large proportion is involved in vacuolar traffic. When mutated, these vacuolar protein-sorting (VPS) genes lead to telomeres shorter than those observed in the wild type. Using genetic analysis, we characterized the pathway by which VPS15, VPS34, VPS22 VPS23 and VPS28 affect the telomeres. Our results indicate that these VPS genes affect telomere length through a single pathway and that this effect requires the activity of telomerase and the Ku heterodimer, but not the activity of Tel1p or Rif2p. We present models to explain the link between vacuolar traffic and telomere length homeostasis.

Original language	English
Pages (from-to)	18-28
Number of pages	11
Journal	Current Genetics
Volume	47
Issue number	1
DOIs	https://doi.org/10.1007/s00294-004-0548-y
State	Published - Jan 2005

Funding

Funders	Funder number
Israel Science Foundation
Ministry of Health, State of Israel

Keywords

ESCRT
Ku heterodimer
Sch9
Telomeres
VPS gene
Vacuole

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10.1007/s00294-004-0548-y

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title = "The yeast VPS genes affect telomere length regulation",

abstract = "Eukaryotic cells invest a large proportion of their genome in maintaining telomere length homeostasis. Among the 173 non-essential yeast genes found to affect telomere length, a large proportion is involved in vacuolar traffic. When mutated, these vacuolar protein-sorting (VPS) genes lead to telomeres shorter than those observed in the wild type. Using genetic analysis, we characterized the pathway by which VPS15, VPS34, VPS22 VPS23 and VPS28 affect the telomeres. Our results indicate that these VPS genes affect telomere length through a single pathway and that this effect requires the activity of telomerase and the Ku heterodimer, but not the activity of Tel1p or Rif2p. We present models to explain the link between vacuolar traffic and telomere length homeostasis.",

keywords = "ESCRT, Ku heterodimer, Sch9, Telomeres, VPS gene, Vacuole",

author = "Ofer Rog and Sarit Smolikov and Anat Krauskopf and Martin Kupiec",

note = "Funding Information: Acknowledgements We would like to thank Y. Mazor, T. Yehuda, H. Maddar and R. Gurevich for their help and support. This work was supported by grants from the Israel Science Foundation (A.K., M.K.) and the Israeli Ministry of Health (M.K.).",

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AU - Smolikov, Sarit

AU - Krauskopf, Anat

AU - Kupiec, Martin

N1 - Funding Information: Acknowledgements We would like to thank Y. Mazor, T. Yehuda, H. Maddar and R. Gurevich for their help and support. This work was supported by grants from the Israel Science Foundation (A.K., M.K.) and the Israeli Ministry of Health (M.K.).

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N2 - Eukaryotic cells invest a large proportion of their genome in maintaining telomere length homeostasis. Among the 173 non-essential yeast genes found to affect telomere length, a large proportion is involved in vacuolar traffic. When mutated, these vacuolar protein-sorting (VPS) genes lead to telomeres shorter than those observed in the wild type. Using genetic analysis, we characterized the pathway by which VPS15, VPS34, VPS22 VPS23 and VPS28 affect the telomeres. Our results indicate that these VPS genes affect telomere length through a single pathway and that this effect requires the activity of telomerase and the Ku heterodimer, but not the activity of Tel1p or Rif2p. We present models to explain the link between vacuolar traffic and telomere length homeostasis.

AB - Eukaryotic cells invest a large proportion of their genome in maintaining telomere length homeostasis. Among the 173 non-essential yeast genes found to affect telomere length, a large proportion is involved in vacuolar traffic. When mutated, these vacuolar protein-sorting (VPS) genes lead to telomeres shorter than those observed in the wild type. Using genetic analysis, we characterized the pathway by which VPS15, VPS34, VPS22 VPS23 and VPS28 affect the telomeres. Our results indicate that these VPS genes affect telomere length through a single pathway and that this effect requires the activity of telomerase and the Ku heterodimer, but not the activity of Tel1p or Rif2p. We present models to explain the link between vacuolar traffic and telomere length homeostasis.

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KW - Ku heterodimer

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The yeast VPS genes affect telomere length regulation

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Keywords

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