The use of cardiolipin-containing liposomes as a model system to study the interaction between proteins and the inner mitochondrial membrane

Milit Marom, Abdussalam Azem

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations

Abstract

The interaction of proteins with biological membranes is a key factor in their biogenesis and proper function. Hence, unraveling the properties of this interaction is very important and constitutes an essential step in deciphering the structural and functional characteristics of a membrane protein. Here we describe the use of cardiolipin-containing liposomes to analyze the interaction of the import protein Tim44 with the inner mitochondrial membrane. Using this system we showed that Tim44 is peripherally attached to the membrane and we detected the membrane binding site of the protein. The cardiolipin-containing liposomes serve as an excellent in vitro model system to the inner mitochondrial membrane and thus provide a good tool to analyze the interaction of various mitochondrial proteins with the inner membrane.

Original languageEnglish
Title of host publicationMembrane Biogenesis
Subtitle of host publicationMethods and Protocols
EditorsDoron Rapaport, Johannes Herrmann
Pages147-155
Number of pages9
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1033
ISSN (Print)1064-3745

Keywords

  • Cardiolipin
  • Inner mitochondrial membrane
  • Liposomes
  • Mitochondria
  • TIM23 import complex
  • Tim44

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