The use of an electric freezer in human semen banking

G. Paz, L. Yogev, A. Gottreich, R. Rotem, H. Yavetz, Z. T. Homonnai

Research output: Contribution to journalArticlepeer-review

Abstract

The use of an electric freezer for cryostorage of human semen is described. A simple method for semen freezing in liquid nitrogen after dilution with 7.5% glycerol was used. Thawing for quality analysis revealed only a small decrease (10%) in post-thaw sperm motility (59 ± 1.4 vs. 53 ± 1.8%; mean ± SE, n = 12, P < 10-5) and 14% in post-thaw sperm vitality (85.0 ± 1.3 vs. 72.9 ± 1.8%). After the freezing process, the samples, three of each donor, were cryopreserved in a regular electric freezer which maintained temperatures in the range of -85 °C (±2 °C). The samples were stored for 1 week, 2 and 6 months, thawed and then assayed for motility and vitality. No effect of storage was found for a period up to 2 months. An additional decrease of 17.2% in sperm motility and 18% in sperm vitality were noted only after 6 months of preservation. The final motility and vitality rates of these sperm samples were 44 ± 2.4 and 60 ± 3.0%, respectively. According to these results, in cases of sperm storage for limited periods, it is recommended to cryopreserve human semen by the use of a combination of freezing in liquid nitrogen and storage of the samples in an electric freezer at -85 °C.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalEuropean Journal of Obstetrics, Gynecology and Reproductive Biology
Volume38
Issue number2
DOIs
StatePublished - 30 Jan 1991

Keywords

  • Cryopreservation
  • Freezing
  • Protective medium
  • Sperm

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