The Ultrastructural Visualization of Cell Surface Glycoconjugates

Edward A. Bayer, Ehud Skutelsky, Meir Wilchek

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

This chapter summarizes approaches and provides representative examples for the selective visualization of specific cell surface saccharides. Over the years, numerous experimental approaches have been instituted for the ultrastructural localization of cell surface polysaccharide coats. For the sake of simplicity, these approaches are divided into three major categories: (1) direct localization, whereby the marker used either possesses innate affinity toward a given surface saccharide or has been chemically altered with an appropriate sugar-specific binder, (2) indirect localization, whereby the interaction of a given marker with a cell surface saccharide depends upon the noncovalent mediation or bridging between the surface site and electron dense marker, and (3) component modified localization whereby the cell surface saccharide is altered by chemical or enzymic means and the modified saccharide can then be specifically localized either directly or indirectly.

Original languageEnglish
Pages (from-to)195-215
Number of pages21
JournalMethods in Enzymology
Volume83
Issue numberC
DOIs
StatePublished - 1 Jan 1982

Funding

FundersFunder number
National Institutes of Health Public Health ServiceF32-ES5120
Leukemia Research Foundation

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