The transduction of mechanical force into biochemical events in bone cells may involve activation of phospholipase A2

Itzhak Binderman, Uriel Zor, Alvin M. Kaye, Zvi Shimshoni, Arie Harell, Dalia Sömjen

Research output: Contribution to journalArticlepeer-review

Abstract

Mechanical forces applied to cultured bone cells induce the production of cAMP via stimulation of the formation of prostaglandin E2 (PGE2) and its release into the medium, resulting in stimulation of adenylate cyclase. In this paper we show that either the antibiotic gentamycin (100 μg/ml) or antiphospholipid antibodies (0.1%) which bind to membrane phospholipids abolish cAMP formation induced by mechanical forces; exogenously added arachidonic acid or PGE2 stimulates cAMP formation, even in the presence of these agents. Addition of exogenous phospholipase A2 (but not phospholipase C) causes an increase in the formation of cAMP in bone cells, a response that is also inhibited by gentamycin or antiphospholipase antibodies. These observations suggest that mechanical forces exert their effect on bone cells via the following chain of events: (1) activation of phospholipase A2, (2) release of arachidonic acid, (3) increased PGE synthesis, (4) augmented cAMP production.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalCalcified Tissue International
Volume42
Issue number4
DOIs
StatePublished - Jul 1988

Keywords

  • Antiphospholipid antibodies
  • Arachidonic acid
  • Bone cell cultures
  • Gentamycin
  • Phospholipase A
  • Prostaglandin E
  • cAMP

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