The Tat protein of the human immunodeficiency virus type 1 (HIV-1) interacts with the EGF-like repeats of the Notch proteins and the EGF precursor

Nitza Shoham, Limor Cohen, Abraham Yaniv, Arnona Gazit

Research output: Contribution to journalArticlepeer-review

Abstract

Employing the yeast two-hybrid system, the Tat protein of the human immunodeficiency virus (HIV) was shown to interact with a region spanning the EGF-like repeats 1-6 of the mouse Notch1, the human Notch2 and the Drosophila Notch. This observation was confirmed in mammalian cells by demonstrating an interaction between the HIV Tat and the EGF-like repeats 1-6 of the various Notch proteins. The HIV Tat protein interacted also with the full-length mouse Notch1 receptor when co-expressed in mammalian cells. Moreover, the HIV Tat protein interacted also with the EGF-like repeats 1-4-spanning domain of the human EGF precursor. The ability of the HIV Tat protein to interact with the Notch proteins and possibly with other EGF-like repeats-bearing proteins, suggests that such interactions might modulate their physiological functions, thus affecting various AIDS-associated pathologies.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalVirus Research
Volume98
Issue number1
DOIs
StatePublished - Dec 2003

Keywords

  • EGF-like repeats
  • HIV
  • Human immunodeficiency virus
  • Notch
  • Tat protein

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