The synergistic tumoricidal activity of anticancer drugs and oxidative burst-triggered macrophages

Simcha Marcovitch; Yona Keisari

doi:10.1007/BF00205577

The synergistic tumoricidal activity of anticancer drugs and oxidative burst-triggered macrophages

Simcha Marcovitch^*, Yona Keisari

^*Corresponding author for this work

Clinical Microbiology and Immunology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The anticancer drugs adriamycin (ADR) and actinomycin D (AMD) were tested for their effect on the oxidative burst (OB) of mouse peritoneal macrophages (MPM) and on the killing of tumor cells by OB-stimulated MPM. The oxidative burst of MPM determined by hydrogen peroxide (H₂O₂) production was severely impaired by ADR (10 μg/ml) and AMD (40 μg/ml) after a 1 h treatment and by lower concentrations of the drugs following a 24 h treatment. The toxicity of the drugs against MPM was comparable to their effect on EL4 cells. Pretreatment of EL4 and TLX-9 tumor cells with sublethal amounts of ADR for 4 h rendered the cells sensitive to the cytotoxic effect of OB-stimulated MPM which were otherwise unable to kill these cells. It seems that anticancer drugs and OB-stimulated macrophages can cooperate in the destruction of tumor cells in vitro.

Original language	English
Pages (from-to)	205-208
Number of pages	4
Journal	Cancer Immunology, Immunotherapy
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/BF00205577
State	Published - Dec 1985

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abstract = "The anticancer drugs adriamycin (ADR) and actinomycin D (AMD) were tested for their effect on the oxidative burst (OB) of mouse peritoneal macrophages (MPM) and on the killing of tumor cells by OB-stimulated MPM. The oxidative burst of MPM determined by hydrogen peroxide (H2O2) production was severely impaired by ADR (10 μg/ml) and AMD (40 μg/ml) after a 1 h treatment and by lower concentrations of the drugs following a 24 h treatment. The toxicity of the drugs against MPM was comparable to their effect on EL4 cells. Pretreatment of EL4 and TLX-9 tumor cells with sublethal amounts of ADR for 4 h rendered the cells sensitive to the cytotoxic effect of OB-stimulated MPM which were otherwise unable to kill these cells. It seems that anticancer drugs and OB-stimulated macrophages can cooperate in the destruction of tumor cells in vitro.",

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The synergistic tumoricidal activity of anticancer drugs and oxidative burst-triggered macrophages

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