The Specificity of Antisera to Conjugates of Prostaglandins E with Bovine Serum Albumin and Thyroglobulin

Amiram RAZ; Michal SCHWARTZMAN; Rina KENIG‐WAKSHAL; Ethel PERL

doi:10.1111/j.1432-1033.1975.tb04051.x

The Specificity of Antisera to Conjugates of Prostaglandins E with Bovine Serum Albumin and Thyroglobulin

Amiram RAZ^*, Michal SCHWARTZMAN, Rina KENIG‐WAKSHAL, Ethel PERL

^*Corresponding author for this work

Biochemistry Molecular Biology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Sera to conjugates of prostaglandins E₁ and E₂ with bovine serum albumin and bovine thyro‐globulin were raised in rabbits to determine their specificity for these prostaglandins. Antisera to bovine serum albumin conjugates of prostaglandins E₁ and E₂ were more reactive against prostaglandins A and B respectively. In contrast, the sera prepared with conjugates of thyroglobulin with prostaglandins E₁ and E₂ displayed marked specificity for the homologous antigens and only minimal cross‐reactivity with prostaglandins A and B respectively. These latter antisera were used to develop a sensitive radioimmunoassay for the E prostaglandins.

Original language	English
Pages (from-to)	145-150
Number of pages	6
Journal	European Journal of Biochemistry
Volume	53
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1975.tb04051.x
State	Published - Apr 1975

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title = "The Specificity of Antisera to Conjugates of Prostaglandins E with Bovine Serum Albumin and Thyroglobulin",

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AU - KENIG‐WAKSHAL, Rina

AU - PERL, Ethel

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N2 - Sera to conjugates of prostaglandins E1 and E2 with bovine serum albumin and bovine thyro‐globulin were raised in rabbits to determine their specificity for these prostaglandins. Antisera to bovine serum albumin conjugates of prostaglandins E1 and E2 were more reactive against prostaglandins A and B respectively. In contrast, the sera prepared with conjugates of thyroglobulin with prostaglandins E1 and E2 displayed marked specificity for the homologous antigens and only minimal cross‐reactivity with prostaglandins A and B respectively. These latter antisera were used to develop a sensitive radioimmunoassay for the E prostaglandins.

AB - Sera to conjugates of prostaglandins E1 and E2 with bovine serum albumin and bovine thyro‐globulin were raised in rabbits to determine their specificity for these prostaglandins. Antisera to bovine serum albumin conjugates of prostaglandins E1 and E2 were more reactive against prostaglandins A and B respectively. In contrast, the sera prepared with conjugates of thyroglobulin with prostaglandins E1 and E2 displayed marked specificity for the homologous antigens and only minimal cross‐reactivity with prostaglandins A and B respectively. These latter antisera were used to develop a sensitive radioimmunoassay for the E prostaglandins.

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The Specificity of Antisera to Conjugates of Prostaglandins E with Bovine Serum Albumin and Thyroglobulin

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