The small GTPase BcCdc42 affects nuclear division, germination and virulence of the gray mold fungus Botrytis cinerea

Leonie Kokkelink, Anna Minz, Muhamad Al-Masri, Sabine Giesbert, Radwan Barakat, Amir Sharon, Paul Tudzynski

Research output: Contribution to journalArticlepeer-review

Abstract

The small GTPase Cdc42 plays a central role in various processes in eukaryotic cells including growth, differentiation and cytoskeleton organization. Whereas it is essential in the yeast Saccharomyces cerevisiae, its role in filamentous fungi differs, due to the complementing, partly overlapping function of Rac. We analyzed the role of the Cdc42 homologue in the necrotrophic, broad host range pathogen Botrytis cinerea. Deletion mutants of bccdc42 showed various growth abnormalities; the mutants had reduced growth rate and hyphal branching, they produced fewer conidia, which were enlarged and misshapen and had germination defects. Additionally, the mutants were impaired in sclerotia development. Cytological studies indicate that at least part of this phenotype could be attributed to disturbed control of nuclear division: conidia and hyphae of the mutant showed twofold higher nucleus/cytoplasm ratio compared to wild type cells. Apart from these effects on vegetative growth and differentiation, Δbccdc42 strains were attenuated in penetration and colonization of host tissue, confirming that BcCdc42 - though being not essential like in yeast - is involved in important developmental processes in B. cinerea.

Original languageEnglish
Pages (from-to)1012-1019
Number of pages8
JournalFungal Genetics and Biology
Volume48
Issue number11
DOIs
StatePublished - Nov 2011

Keywords

  • Cell cycle
  • G-proteins
  • Host-pathogen-interaction
  • Pathogenic fungi

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