The single cell as a tool for genetic testing: Credibility, precision, implication

Keren Dotan, Baruch Feldman, Boleslaw Goldman, Yehuda Peri, Leah Peleg

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To investigate the influence of amplicons size and cell type on allele dropout and amplification failures in single-cell based molecular diagnosis. Methods: 730 single lymphocytes and amniotic cells were collected from known heterozygotes individuals to one of the common Ashkenazi Jewish mutations: 1278+TATC and IVS12+1G>C which cause Tay Sachs Disease, IVS20+6T and 854A>C which underlie Familial Dysautonomia and Canavan Disease. DNA was extracted and analyzed by our routine methods. Results: Reduced rates of allele dropout and amplification failure were found when smaller amplification product were designed and in amniotic cultured cells compared to peripheral lymphocytes. Cultured lymphocytes, induced to divide, demonstrated significantly less allele dropout than non induced lymphocytes suggesting the role of division potential on amplification efficiency. Conclusion: Single cell based diagnosis should be designed for each mutation. Minimal sized amplicons and cell having division potential should be preferred, as well as sensitive techniques to detect preferential amplification.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume27
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • Allele drop-out
  • PCR-failure
  • Preimplantation genetic diagnosis
  • Single cell

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