TY - JOUR
T1 - The role of superoxide dismutation in malaria parasites
AU - Schwartz, Eli
AU - Samuni, Amram
AU - Friedman, Ilanit
AU - Hempelmann, Ernest
AU - Golenser, Jacob
N1 - Funding Information:
Acknowledgments—This research was supported by Binational Science Foundation and by grant from the Beryl grant 95–00287 from the USA-Israel and Francess WeinsteinFoundation.
PY - 1999
Y1 - 1999
N2 - Oxidant stress is associated with the generation of reactive oxygen species that are responsible for the damage of a variety of cellular components. The prevention of such biological damage can be achieved by dismutation of superoxide to H2O2 which in turn is removed by catalase and GSH peroxidase. However, redox-active iron released during the development of plasmodia in the erythrocyte can mediate the conversion of H2O2 to hydroxyl radical which is more reactive. The roles of SOD and the nitroxide SOD mimic 4-OH,2,2,6,6,tetramethyl piperidine-N-oxyl (Tempol) were examined in P. falciparum grown in vitro. Both compounds did not prevent the interference with growth inflicted by various inducers of oxidant stress. Moreover. Tempol inhibited parasite growth, in agreement with previous experiments depicting accelerated mortality in SOD overexpressing mouse model of malaria. Probably, effective defense against ROS requires balanced increments in antioxidant enzymes and is not necessarily improved by an increase in the activity of one enzyme.
AB - Oxidant stress is associated with the generation of reactive oxygen species that are responsible for the damage of a variety of cellular components. The prevention of such biological damage can be achieved by dismutation of superoxide to H2O2 which in turn is removed by catalase and GSH peroxidase. However, redox-active iron released during the development of plasmodia in the erythrocyte can mediate the conversion of H2O2 to hydroxyl radical which is more reactive. The roles of SOD and the nitroxide SOD mimic 4-OH,2,2,6,6,tetramethyl piperidine-N-oxyl (Tempol) were examined in P. falciparum grown in vitro. Both compounds did not prevent the interference with growth inflicted by various inducers of oxidant stress. Moreover. Tempol inhibited parasite growth, in agreement with previous experiments depicting accelerated mortality in SOD overexpressing mouse model of malaria. Probably, effective defense against ROS requires balanced increments in antioxidant enzymes and is not necessarily improved by an increase in the activity of one enzyme.
UR - http://www.scopus.com/inward/record.url?scp=0032837657&partnerID=8YFLogxK
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C2 - 10443798
AN - SCOPUS:0032837657
SN - 0360-3997
VL - 23
SP - 361
EP - 370
JO - Inflammation
JF - Inflammation
IS - 4
ER -
