TY - JOUR
T1 - The role of lysines in Euglena cytochrome c-552. Chemical modification studies
AU - Aviram, Irit
N1 - Funding Information:
I am grateful to Dr. G. W. Pettigrew for his advice and help in the course of this work. This work was supported by Research Grant 295 of the United States-Israel Binational Science Foundation, Jerusalem.
PY - 1977/5
Y1 - 1977/5
N2 - Cytochrome c-552 of Euglena gracilis was modified by transamination of the terminal amino groups and by acetylation and maleylation of α- and ε{lunate}-amino groups. The properties of the derivatives were studied with respect to their optical spectra, hemelinked ionizations, reductions with ascorbate, redox potential, and ability to participate in light-dependent electron transfer. Transaminated cytochrome c-552 resembled the native protein. Acetylation and maleylation did not lead to marked conformation changes but altered the crevice structure. At alkaline pH, acetylated and maleylated cytochrome c-552 existed in a mixed spin form. Both the reduction rates with ascorbate as well as the light-dependent oxidation rates were lowered by these modifications.
AB - Cytochrome c-552 of Euglena gracilis was modified by transamination of the terminal amino groups and by acetylation and maleylation of α- and ε{lunate}-amino groups. The properties of the derivatives were studied with respect to their optical spectra, hemelinked ionizations, reductions with ascorbate, redox potential, and ability to participate in light-dependent electron transfer. Transaminated cytochrome c-552 resembled the native protein. Acetylation and maleylation did not lead to marked conformation changes but altered the crevice structure. At alkaline pH, acetylated and maleylated cytochrome c-552 existed in a mixed spin form. Both the reduction rates with ascorbate as well as the light-dependent oxidation rates were lowered by these modifications.
UR - http://www.scopus.com/inward/record.url?scp=0017404661&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(77)90498-2
DO - 10.1016/0003-9861(77)90498-2
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AN - SCOPUS:0017404661
SN - 0003-9861
VL - 181
SP - 199
EP - 207
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -