The role of a tyrosyl residue in the mechanism of action of carboxypeptidase B: luminescence studies

N. Shaklai, N. Zisapel, M. Sokolovsky

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The luminescence spectra of carboxypeptidase B indicate specific differences between the zinc and apoenzyme due to the state of tyrosyl residues presumably at the active site. These differences disappear when enzyme substrate or enzyme inhibitor complexes are formed, suggesting that they may reflect the interaction of a tyrosyl residue in the native enzyme with the catalytically essential zinc atom. An interpretation of the role of that tyrosyl residue in the mechanism of action of carboxypeptidase B is presented.

Original languageEnglish
Pages (from-to)2025-2028
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume70
Issue number7
DOIs
StatePublished - 1973

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