The regulatory network of Vibrio parahaemolyticus type VI secretion system 1

Rotem Ben-Yaakov, Dor Salomon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Type VI secretion systems (T6SSs) are widespread, tightly regulated, protein delivery apparatuses used by Gram-negative bacteria to outcompete their neighbours. The pathogen, Vibrio parahaemolyticus, encodes two T6SSs. These T6SSs are differentially regulated by external conditions. T6SS1, an antibacterial system predominantly found in pathogenic isolates, requires warm marine-like conditions and surface sensing for activation. The regulatory network that governs this activation is not well understood. In this work, we devised a screening methodology that allows us to easily monitor the outcome of bacterial competitions and thus to identify mutants that are defective in T6SS1-mediated bacterial killing. The methodology, termed Bacterial Competition Fluorescence (BaCoF), relies on detection of a fluorescent signal as an indicator of the survival and growth of a T6SS-sensitive, GFP-expressing prey that has been co-cultured with mutants derived from a T6SS+ attacker of interest. Using BaCoF, we screened a random transposon insertion mutant library and identified genes required for V. parahaemolyticus T6SS1 activation, among them TfoY and Tmk. We used epistasis experiments to determine the relationships between the newly identified components and other regulators that were previously described. Thus, we present here a detailed biological understanding of the T6SS1 regulatory network.

Original languageEnglish
Pages (from-to)2248-2260
Number of pages13
JournalEnvironmental Microbiology
Issue number7
StatePublished - Jul 2019


FundersFunder number
European Union's Horizon 2020
Horizon 2020 Framework Programme714224
European Research Council
Israel Science Foundation920/17


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