The reculture technique: Individualizing the treatment of Acanthamoeba keratitis

Y. S. Wysenbeek, D. Blank-Porat, N. Harizman, Tamara Wygnanski-Jaffe, N. Keller, I. Avni

Research output: Contribution to journalArticlepeer-review


Purpose. To assess the efficacy of three drugs in different concentrations against different strains of Acanthamoeba using the reculture technique. Methods. Cysts and trophozoites were immersed in five separate solutions. The solutions administered included 0.1% and 0.02% polyhexamethylene biguanide (PHMB), 0.1% and 0.02% chlorhexidine, and propamidine isethionate (Brolene). Readings took place after 1, 5, and 24 hours. The cysts and trophozoites were then recultured for an additional period of 48 hours. An effective drug was defined as a medication that inhibited any growth of trophozoites using the reculture technique. Results. Chlorhexidine at concentrations of 0.1% and 0.02% was the only effective drug against all five strains of Acanthamoeba examined, and no trophozoites were detected on plates immersed with this agent. Only 0.1% chlorhexidine was effective in destroying all cysts in the five strains examined. Conclusion. We found that 0.02% chlorhexidine was efficient in irradicating all trophozoites and 0.1% chlorhexidine was effective in eradicating all cysts in the samples we examined. Therefore, it may be possible that 0.02% chlorhexidine is a good initial treatment in amoebic keratitis. Sensitivity testing, then, may be performed using the reculture technique and specification of therapy can be made accordingly.

Original languageEnglish
Pages (from-to)464-467
Number of pages4
Issue number4
StatePublished - Jul 2000
Externally publishedYes


  • Acanthamoeba
  • Culture
  • Keratitis
  • Reculture technique


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