The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

Martin Kupiec

doi:10.1007/BF00419878

The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

Martin Kupiec^*

^*Corresponding author for this work

Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The gene RAD50 was located by the ability of subclones to restore the Rad⁺ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad⁺ strains.

Original language	English
Pages (from-to)	487-489
Number of pages	3
Journal	Current Genetics
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/BF00419878
State	Published - Feb 1986
Externally published	Yes

Keywords

DNA repair
Gene disruption

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10.1007/BF00419878

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title = "The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth",

abstract = "The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.",

keywords = "DNA repair, Gene disruption",

author = "Martin Kupiec",

year = "1986",

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doi = "10.1007/BF00419878",

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AU - Kupiec, Martin

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N2 - The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.

AB - The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.

KW - DNA repair

KW - Gene disruption

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JO - Current Genetics

JF - Current Genetics

IS - 6

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The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

Abstract

Keywords

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