The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

Research output: Contribution to journalArticlepeer-review

Abstract

The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.

Original languageEnglish
Pages (from-to)487-489
Number of pages3
JournalCurrent Genetics
Volume10
Issue number6
DOIs
StatePublished - Feb 1986
Externally publishedYes

Keywords

  • DNA repair
  • Gene disruption

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