Abstract
The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.
Original language | English |
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Pages (from-to) | 487-489 |
Number of pages | 3 |
Journal | Current Genetics |
Volume | 10 |
Issue number | 6 |
DOIs | |
State | Published - Feb 1986 |
Externally published | Yes |
Keywords
- DNA repair
- Gene disruption