The putative role of arylsulfatase in interleukin-2-mediated cytotoxicity and interleukin-7-mediated bactericidal activity of natural killer cells

A. Toren, M. Gadish, I. Fabian, A. Nagler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have examined the cytolytic and bactericidal activity of resting and cytokine-stimulated natural-killer (NK) cells against K562 and Daudi cell lines and Escherichia coli, respectively. Unstimulated NK cells showed considerable cytolytic activity against K562 (64 ± 4%) and relatively low activity against the Daudi cell lines (22 ± 9%). Pretreatment of NK cells with the arylsulfatase (AS) type-II-specific inhibitor NaH2PO4 reduced cytotoxicity towards K562 and Daudi 1.3- and 2.9-fold (p < 0.05; n = 12), respectively, indicating that AS participates in NK-mediated cytotoxicity. Interleukin-2 (IL-2) (200 units/ml) caused a 1.3- and 3.5-fold (p < 0.5; n = 12) enhancement of NK cytotoxic activity against K562 and Daudi, respectively. Pretreatment of these cells with the AS type-II-specific inhibitor NaH2PO4 reduced cytotoxicity 1.1-fold towards K562 (p > 0.05; n = 12) and 1.2-fold towards Daudi (p > 0.05; n = 12) indicating that AS does not participate in IL-2-mediated NK cytolytic activity against these cell lines. IL-7 (3 units/ml) did not cause any enhancement of NK cytolytic activity. Unstimulated NK cells showed considerable bactericidal activity against E. coli (23 ± 4%). Incubation of resting NK cells with NaH2PO4 reduced the bactericidal effect only by 1.09-fold (p > 0.05; n = 12), indicating that AS does not mediate this effect. IL-2 (200 units/ml) and IL-7 (3 units/ml) enhanced the bactericidal activity 1.5- and 2.2-fold, respectively (p < 0.05; n = 12). This effect was not influenced by incubation of IL-2-stimulated cells with NaH2PO4, indicating that AS does not participate in the IL-2-mediated NK bactericidal effect. IL-2 seems to exert its stimulatory effect upon NK-mediated bactericidal activity by a different, non-AS-dependent mechanism. However, incubation of IL-7-stimulated NK cells with NaH2PO4 reduced the NK bactericidal effect by 1.2-fold (p < 0.05; n = 12), indicating that AS may have a role in this reaction. These data can be further confirmed by detection of AS through degranulation of NK cells, showing that IL-2 induced only mild degranulation of resting and f-MLP-stimulated NK cells (26 ± 1% vs 22 ± 2% and 31 ± 2% vs 29 ± 2, respectively) (p > 0.05; n = 8). In contrast, IL-7 showed significant enhancement of AS release in resting or f-MLP-induced NK cells (36 ± 3% vs 22 ± 2% and 49 ± 3% vs 29 ± 2%, respectively) (p < 0.05; n = 8).

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalAnnals of Hematology
Volume74
Issue number2
DOIs
StatePublished - 1997

Keywords

  • Arylsulfatase
  • Bactericidal
  • Cytotoxic
  • Degranulation
  • Interleukin 2
  • Interleukin 7
  • NK cells

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