TY - JOUR
T1 - The phospholipase c β3 gene located in the MEN1 region shows loss of expression in endocrine tumours
AU - Weber, Günther
AU - Friedman, Eitan
AU - Grimmond, Sean
AU - Hayward, Nicholas K.
AU - Phelan, Catherine
AU - Skogseid, Britt
AU - Gobl, Anders
AU - Zedenius, Jan
AU - Sandelin, Kerstin
AU - Teh, Bin Tean
AU - Carson, Emma
AU - White, Irene
AU - Öberg, Kjell
AU - Shepherd, Joseph
AU - Nordenskjöld, Magnus
AU - Larsson, Catharina
N1 - Funding Information:
This work was supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Axel and Margaret Ax:son Johnson Fund, the Magnus Bergwall Fund, the Lars Hierta Foundation, the Queensland Cancer Fund, and the National Health and Medical Research Council of Australia. N.K.H. is a recipient of an R.D.Wright Fellowship from the National Health and Medical Research Council of Australia.
PY - 1994/10
Y1 - 1994/10
N2 - Oncogenesis of tumours related to multiple endocrine neoplasia type 1 (MEN1) is associated with somatic deletions involving the MEN1 locus, suggesting inactivation of a tumour suppressor gene in this region. Identification of meiotic cross-overs in MEN1 families has placed the MEN1 locus centromeric of D11S807. An extended deletion mapping was performed in 27 primary parathyroid tumours, and identified D11S427 as the closest centromeric flanking marker. Through physical mapping using newly isolated cDNA clones, we estimated the distance between the flanking markers D11S807 and D11S427 to be less than 900 kb. One of these cDNA clones showed expression of a 4.4 kb message in multiple tissues, including those affected in MEN1, while in five endocrine tumours no transcript was detected. Sequence characterization showed that this gene encodes for the phospholipase C β3, a key enzyme in signal transduction.
AB - Oncogenesis of tumours related to multiple endocrine neoplasia type 1 (MEN1) is associated with somatic deletions involving the MEN1 locus, suggesting inactivation of a tumour suppressor gene in this region. Identification of meiotic cross-overs in MEN1 families has placed the MEN1 locus centromeric of D11S807. An extended deletion mapping was performed in 27 primary parathyroid tumours, and identified D11S427 as the closest centromeric flanking marker. Through physical mapping using newly isolated cDNA clones, we estimated the distance between the flanking markers D11S807 and D11S427 to be less than 900 kb. One of these cDNA clones showed expression of a 4.4 kb message in multiple tissues, including those affected in MEN1, while in five endocrine tumours no transcript was detected. Sequence characterization showed that this gene encodes for the phospholipase C β3, a key enzyme in signal transduction.
UR - http://www.scopus.com/inward/record.url?scp=0028170327&partnerID=8YFLogxK
U2 - 10.1093/hmg/3.10.1775
DO - 10.1093/hmg/3.10.1775
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C2 - 7849701
AN - SCOPUS:0028170327
SN - 0964-6906
VL - 3
SP - 1775
EP - 1781
JO - Human Molecular Genetics
JF - Human Molecular Genetics
IS - 10
ER -