The Oxidative Burst and Related Phenomena in Mouse Macrophages Elicited by Different Sterile Inflammatory Stimuli

Y. Keisari; Lilian Braun; E. Flescher

doi:10.1016/S0171-2985(83)80048-5

The Oxidative Burst and Related Phenomena in Mouse Macrophages Elicited by Different Sterile Inflammatory Stimuli

Y. Keisari^*, Lilian Braun, E. Flescher

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O₂⁻) and hydrogen peroxide (H₂O₂) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan, and wheat-germ agglutinin (WGA) were found to stimulate O₂⁻ and H₂O₂ production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of ¹²⁵I. Macrophage-mediated cytolysis of ⁵¹Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H₂O₂ in the lytic process.

Original language	English
Pages (from-to)	78-89
Number of pages	12
Journal	Immunobiology
Volume	165
Issue number	1
DOIs	https://doi.org/10.1016/S0171-2985(83)80048-5
State	Published - 1983

Keywords

12-O-tetradecanoyl-phorbol-13-acetate
BSS
ConA
Eagle's minimal essential medium
Earle's balanced salt solution
FCS
FMLPA
HRP
MEM
MMC
MPM
PO
SOD
TG
TPA
WGA
concanavalin A
fetal calf serum
formyl methionyl leucyl phenylalanine
horseradish peroxidase
macrophage-mediated cytolysis
mouse peritoneal macrophages
paraffin oil
superoxide dismutase
thioglycollate
wheat-germ agglutinin

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10.1016/S0171-2985(83)80048-5

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title = "The Oxidative Burst and Related Phenomena in Mouse Macrophages Elicited by Different Sterile Inflammatory Stimuli",

abstract = "Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O2−) and hydrogen peroxide (H2O2) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan, and wheat-germ agglutinin (WGA) were found to stimulate O2− and H2O2 production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of 125I. Macrophage-mediated cytolysis of 51Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H2O2 in the lytic process.",

keywords = "12-O-tetradecanoyl-phorbol-13-acetate, BSS, ConA, Eagle's minimal essential medium, Earle's balanced salt solution, FCS, FMLPA, HRP, MEM, MMC, MPM, PO, SOD, TG, TPA, WGA, concanavalin A, fetal calf serum, formyl methionyl leucyl phenylalanine, horseradish peroxidase, macrophage-mediated cytolysis, mouse peritoneal macrophages, paraffin oil, superoxide dismutase, thioglycollate, wheat-germ agglutinin",

author = "Y. Keisari and Lilian Braun and E. Flescher",

year = "1983",

doi = "10.1016/S0171-2985(83)80048-5",

language = "אנגלית",

volume = "165",

pages = "78--89",

journal = "Immunobiology",

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T1 - The Oxidative Burst and Related Phenomena in Mouse Macrophages Elicited by Different Sterile Inflammatory Stimuli

AU - Keisari, Y.

AU - Braun, Lilian

AU - Flescher, E.

PY - 1983

Y1 - 1983

N2 - Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O2−) and hydrogen peroxide (H2O2) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan, and wheat-germ agglutinin (WGA) were found to stimulate O2− and H2O2 production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of 125I. Macrophage-mediated cytolysis of 51Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H2O2 in the lytic process.

AB - Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O2−) and hydrogen peroxide (H2O2) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan, and wheat-germ agglutinin (WGA) were found to stimulate O2− and H2O2 production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of 125I. Macrophage-mediated cytolysis of 51Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H2O2 in the lytic process.

KW - 12-O-tetradecanoyl-phorbol-13-acetate

KW - BSS

KW - ConA

KW - Eagle's minimal essential medium

KW - Earle's balanced salt solution

KW - FCS

KW - FMLPA

KW - HRP

KW - MEM

KW - MMC

KW - MPM

KW - PO

KW - SOD

KW - TG

KW - TPA

KW - WGA

KW - concanavalin A

KW - fetal calf serum

KW - formyl methionyl leucyl phenylalanine

KW - horseradish peroxidase

KW - macrophage-mediated cytolysis

KW - mouse peritoneal macrophages

KW - paraffin oil

KW - superoxide dismutase

KW - thioglycollate

KW - wheat-germ agglutinin

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DO - 10.1016/S0171-2985(83)80048-5

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AN - SCOPUS:0020575250

SN - 0171-2985

VL - 165

SP - 78

EP - 89

JO - Immunobiology

JF - Immunobiology

IS - 1

ER -

The Oxidative Burst and Related Phenomena in Mouse Macrophages Elicited by Different Sterile Inflammatory Stimuli

Abstract

Keywords

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