TY - JOUR
T1 - The neuroprotective adenosine-activated signal transduction pathway involves activation of phospholipase C
AU - Rogel, A.
AU - Bromberg, Y.
AU - Sperling, O.
AU - Zoref-Shani, E.
PY - 2006/6/1
Y1 - 2006/6/1
N2 - We have demonstrated before that exposure of neuronal cultures to poisoning by iodoacetic acid (IAA) followed by "reperfusion" (IAA-R insult), results in severe cytotoxicity, which could be markedly attenuated by prior activation of the adenosine A1 receptors. We also have demonstrated that adenosine activates a signal transduction pathway (STP), which involves activation of PKCε and opening of KATP channels. Here, we provide proof for the involvement also of phospholipase C (PLC) in the neuronal protective adenosine-activated STP. R-PIA, a specific A1 adenosine receptor agonist, was found to enhance neuronal PLC activity and protect against the IAA-R insult. The PLC inhibitor U73122, abrogated both R-PIA-induced effects. These results demonstrate that activation of PLC is a vital step in the neuronal protective adenosine-induced STP.
AB - We have demonstrated before that exposure of neuronal cultures to poisoning by iodoacetic acid (IAA) followed by "reperfusion" (IAA-R insult), results in severe cytotoxicity, which could be markedly attenuated by prior activation of the adenosine A1 receptors. We also have demonstrated that adenosine activates a signal transduction pathway (STP), which involves activation of PKCε and opening of KATP channels. Here, we provide proof for the involvement also of phospholipase C (PLC) in the neuronal protective adenosine-activated STP. R-PIA, a specific A1 adenosine receptor agonist, was found to enhance neuronal PLC activity and protect against the IAA-R insult. The PLC inhibitor U73122, abrogated both R-PIA-induced effects. These results demonstrate that activation of PLC is a vital step in the neuronal protective adenosine-induced STP.
KW - Adenosine
KW - Iodoacetic acid
KW - Neuroprotection
KW - Phospholipase C (PLC)
KW - Signal transduction pathway
KW - U73122
UR - http://www.scopus.com/inward/record.url?scp=33750488163&partnerID=8YFLogxK
U2 - 10.1080/15257770600890939
DO - 10.1080/15257770600890939
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AN - SCOPUS:33750488163
SN - 1525-7770
VL - 25
SP - 1283
EP - 1286
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 9-11
ER -