The murine telomerase catalytic subunit shares the PAb-240 mutant specific epitope of the p53 protein

Igor Shats, Michael Milyavsky, Neta Erez, Varda Rotter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Many tumorigenic p53 mutants gain a common antigenic epitope that is recognized by the PAb-240 antibody. Database search identified the presence of this epitope in several other proteins, including several antibodies and the catalytic subunit of mouse telomerase, mTERT. These antibodies may represent a part of the previously demonstrated anti-idiotypic network built around p53. In the present study we demonstrate that the PAb-240 antibody was able to inhibit telomerase activity in extracts from both mouse and human tumor cells. The recognition of mTERT by PAb-240 is demonstrated by Western blotting and by using blocking peptides derived from mTERT. The existence of a shared epitope between mutant p53 and telomerase may suggest that the two proteins contribute to malignant transformation through a common pathway.

Original languageEnglish
Pages (from-to)321-324
Number of pages4
JournalFEBS Letters
Volume546
Issue number2-3
DOIs
StatePublished - 10 Jul 2003
Externally publishedYes

Funding

FundersFunder number
German-Israeli Foundation for Scientific Research and Development
United States-Israel Binational Science Foundation

    Keywords

    • Epitope
    • Mutant p53
    • PAb-240
    • Telomerase

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