The monoclonality of human B-cell lymphomas*

Ronald Levy, Roger Warnke, Ronald F. Dorfman, Joseph Haimovich

Research output: Contribution to journalArticlepeer-review


Human tissues involved with lymphoma have been examined in frozen sections for immunoglobulin-bearing cells by a technique involving double-label immunofluorescence with mixed anti-κ and anti-λ antibodies. F(ab’)2 fragments of purified antibodies were employed to avoid any binding via Fc receptors. B cell lymphomas were shown to be composed of monoclonal populations of Igbearing cells, whereas normal or reactive lymphoid follicles contained a mosaic of Ig-bearing cells derived from multiple clones. Nodules of lymphoma were often surrounded by normal polyclonal B cell populations. We anticipate that the approach described here will be useful in the diagnosis of lymphoma, differentiating it from reactive lymphoid hyperplasia by the demonstration of monoclonality. In addition, it should provide a sensitive and reliable tool for investigating the immunobiology of human lymphoma.

Original languageEnglish
Pages (from-to)1014-1028
Number of pages15
JournalJournal of Experimental Medicine
Issue number4
StatePublished - 1 Apr 1977
Externally publishedYes


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