The molecular dynamics of osteoclast adhesions

Chen Luxenburg, Lia Addadi, Benjamin Geiger

Research output: Contribution to journalArticlepeer-review

Abstract

Podosomes are specialized adhesive structures that play a central role in bone resorption. In this article we address the molecular diversity and dynamics of podosomes at different states of organization, ranging from scattered distribution over the entire ventral membrane of non-polarized cells, via formation of podosome clusters and developing rings to the assembly of a peripheral belt, resembling the sealing zone of polarized, bone-resorbing osteoclasts. Based on published data and on our own results, we describe here the spatial relationships between key podosome-associated proteins. Using quantitative microscopy, we show here a dramatic increase in the local levels of F-actin, vinculin, paxillin, and α-actinin, which occurs upon the transformation of clustered podosomes into rings and sealing zone-like structures. This change is accompanied by a marked decrease in phosphotyrosine levels in the same region. Therefore, our data suggest that a major change in the molecular composition of podosomes is taking place during osteoclast polarization, a change that may be related to adhesion "reinforcement", associated with the assembly of the bone-resorbing apparatus. Studying the nature of the proteins that undergo de-phosphorylation is critical for the understanding of the mechanisms regulating the processes described above.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalEuropean Journal of Cell Biology
Volume85
Issue number3-4
DOIs
StatePublished - 7 Apr 2006
Externally publishedYes

Keywords

  • Bone resorption
  • Cell adhesion
  • Osteoclasts
  • Podosomes
  • Sealing zone
  • Tyrosine phosphorylation

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