TY - JOUR
T1 - The mitochondrial protein translocation motor
T2 - Structural conservation between the human and yeast tim14/pam18- tim16/pam16 co-chaperones
AU - Elsner, Shira
AU - Simian, Dana
AU - Iosefson, Ohad
AU - Marom, Milit
AU - Azem, Abdussalam
PY - 2009/5
Y1 - 2009/5
N2 - Most of our knowledge regarding the process of protein import into mitochondria has come from research employing Saccharomyces cerevisiae as a model system. Recently, several mammalian homologues of the mitochondrial motor proteins were identified. Of particular interest for us is the human Tim14/Pam18-Tim16/Pam16 complex. We chose a structural approach in order to examine the evolutionary conservation between yeast Tim14/Pam18-Tim16/Pam16 proteins and their human homologues. For this purpose, we examined the structural properties of the purified human proteins and their interaction with their yeast homologues, in vitro. Our results show that the soluble domains of the human Tim14/Pam18 and Tim16/Pam16 proteins interact with their yeast counterparts, forming heterodimeric complexes and that these complexes interact with yeast mtHsp70.
AB - Most of our knowledge regarding the process of protein import into mitochondria has come from research employing Saccharomyces cerevisiae as a model system. Recently, several mammalian homologues of the mitochondrial motor proteins were identified. Of particular interest for us is the human Tim14/Pam18-Tim16/Pam16 complex. We chose a structural approach in order to examine the evolutionary conservation between yeast Tim14/Pam18-Tim16/Pam16 proteins and their human homologues. For this purpose, we examined the structural properties of the purified human proteins and their interaction with their yeast homologues, in vitro. Our results show that the soluble domains of the human Tim14/Pam18 and Tim16/Pam16 proteins interact with their yeast counterparts, forming heterodimeric complexes and that these complexes interact with yeast mtHsp70.
KW - MtHsp70
KW - Tim14 (also known as Pam18)
KW - Tim16 (also known as Pam16)
KW - Translocation motor
UR - http://www.scopus.com/inward/record.url?scp=67049108831&partnerID=8YFLogxK
U2 - 10.3390/ijms10052041
DO - 10.3390/ijms10052041
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C2 - 19564938
AN - SCOPUS:67049108831
SN - 1661-6596
VL - 10
SP - 2041
EP - 2053
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 5
ER -