TY - JOUR
T1 - The mitochondrial DNA mutation (ΔmtDNA5286) in human oocytes
T2 - Correlation with age and IVF outcome
AU - Yesodi, Vered
AU - Yaron, Yuval
AU - Lessing, Joseph B.
AU - Amit, Ami
AU - Ben-Yosef, Dalit
PY - 2002
Y1 - 2002
N2 - Purpose: To evaluate the correlation between the presence of a newly described mitochondrial DNA (mtDNA) mutation (ΔmtDNA5286), patients' age, and in vitro fertilization (IVF) outcomes. Methods: The presence of ΔmtDNA5286 was analyzed by nested-primer PCR in 224 unfertilized oocytes of 81 women undergoing IVF for various reasons. Age, number of oocytes retrieved, fertilization and embryo cleavage, number of embryos transferred, and pregnancy rates were compared between patients with and without a mtDNA mutation in their oocytes. Results: The 23 patients in which ΔmtDNA5286 was detected in at least one oocyte were significantly younger than the other 58 with no mutations (30.9 years vs. 33.8 years, respectively, P = 0.03), and had a significantly lower fertilization rate (28.9% vs. 42.1%, respectively, P = 0.01). No other outcome variable was significantly different between the two groups. Conclusion(s): The mtDNA mutation ΔmtDNA5286 may serve as a marker of decreased oocyte quality in IVF.
AB - Purpose: To evaluate the correlation between the presence of a newly described mitochondrial DNA (mtDNA) mutation (ΔmtDNA5286), patients' age, and in vitro fertilization (IVF) outcomes. Methods: The presence of ΔmtDNA5286 was analyzed by nested-primer PCR in 224 unfertilized oocytes of 81 women undergoing IVF for various reasons. Age, number of oocytes retrieved, fertilization and embryo cleavage, number of embryos transferred, and pregnancy rates were compared between patients with and without a mtDNA mutation in their oocytes. Results: The 23 patients in which ΔmtDNA5286 was detected in at least one oocyte were significantly younger than the other 58 with no mutations (30.9 years vs. 33.8 years, respectively, P = 0.03), and had a significantly lower fertilization rate (28.9% vs. 42.1%, respectively, P = 0.01). No other outcome variable was significantly different between the two groups. Conclusion(s): The mtDNA mutation ΔmtDNA5286 may serve as a marker of decreased oocyte quality in IVF.
KW - Fertilization
KW - Human oocytes
KW - Mitochondrial DNA
KW - Mutations
UR - http://www.scopus.com/inward/record.url?scp=0036203281&partnerID=8YFLogxK
U2 - 10.1023/A:1014439529813
DO - 10.1023/A:1014439529813
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AN - SCOPUS:0036203281
SN - 1058-0468
VL - 19
SP - 60
EP - 66
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 2
ER -