The middle subunit of replication protein A contacts growing RNA-DNA primers in replicating simian virus 40 chromosomes

Gilad Mass, Tamar Nethanel, Gabriel Kaufmann

Research output: Contribution to journalArticlepeer-review

Abstract

The eukaryotic single-stranded DNA binding protein replication protein A (RPA) participates in major DNA transactions. RPA also interacts through its middle subunit (Rpa2) with regulators of the cell division cycle and of the response to DNA damage. A specific contact between Rpa2 and nascent simian virus 40 DNA was revealed by in situ UV cross-linking. The dynamic attributes of the cross-linked DNA, namely, its size distribution, RNA primer content, and replication fork polarity, were determined. These data suggest that Rpa2 contacts the early DNA chain intermediates synthesized by DNA polymerase α- primase (RNA-DNA primers) but not more advanced products. Possible signaling functions of Rpa2 are discussed, and current models of eukaryotic lagging- strand DNA synthesis are evaluated in view of our results.

Original languageEnglish
Pages (from-to)6399-6407
Number of pages9
JournalMolecular and Cellular Biology
Volume18
Issue number11
DOIs
StatePublished - Nov 1998

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