The metC gene in Escherichia coli K-12: Isolation and studies of relatedness in Enterobacteriaceae

Shulamit Michaeli, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

Abstract

A specialized transducing phage λ carrying the structural gene for β-cystathionase (metC) of Escherichia coli was isolated. The phage carries a 21-kb fragment of the E. coli K-12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using EcoRI or HindIII. The information for the metC gene is contained in a 1.3-kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalFEMS Microbiology Letters
Volume22
Issue number1
DOIs
StatePublished - 1 Apr 1984

Keywords

  • Methionine
  • cystathionase
  • metC

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