A specialized transducing phage λ carrying the structural gene for β-cystathionase (metC) of Escherichia coli was isolated. The phage carries a 21-kb fragment of the E. coli K-12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using EcoRI or HindIII. The information for the metC gene is contained in a 1.3-kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.
|Number of pages||5|
|Journal||FEMS Microbiology Letters|
|State||Published - 1 Apr 1984|