TY - JOUR
T1 - The mechanism of action of soluble lymphocyte mediators. IV. Effect of migration inhibitory factor (MIF) on macrophage cyclic AMP and on responsiveness to adenylare cyclase stimulators
AU - Pick, Edgar
N1 - Funding Information:
1 This work was supported by Grant 5 ROl AI 11194 from the National Institutes of Health, Bethesda, Maryland; by a grant from the U.S.-Israel Binational Science Foundation; and by a grant from F. Hoffmann-LaRoche & Co. Ltd., Basel, Switzerland.
PY - 1977/8
Y1 - 1977/8
N2 - Intracellular levels of cyclic 3′,5′-adenosine monophosphate (cAMP) in purified guinea pig peritoneal macrophages were elevated following incubation with the adenylate cyclase stimulators prostaglandins E1 and E2 (PGE1, PGE2), isoproterenol, and cholera toxin. Exposure of macrophages to antigen-stimulated lymphocyte culture supernatants, containing migration inhibitory factor (MIF), resulted in a moderate but consistent decrease in the cAMP level, which was best expressed after 1-2 hr of incubation. Incubation of macrophages with MIF-containing supernatants or partially purified MIF for 1-2 hr resulted in reduced cAMP accumulation in response to PGE1, PGE2, isoproterenol, and cholera toxin (nonspecific refractoriness). These findings indicate that MIF-induced inhibition of macrophage migration is not due to an increase in the cellular level of cAMP and that the reduction in cAMP concentration, caused by MIF, is probably a secondary phenomenon unrelated to the inhibition of cellular motility.
AB - Intracellular levels of cyclic 3′,5′-adenosine monophosphate (cAMP) in purified guinea pig peritoneal macrophages were elevated following incubation with the adenylate cyclase stimulators prostaglandins E1 and E2 (PGE1, PGE2), isoproterenol, and cholera toxin. Exposure of macrophages to antigen-stimulated lymphocyte culture supernatants, containing migration inhibitory factor (MIF), resulted in a moderate but consistent decrease in the cAMP level, which was best expressed after 1-2 hr of incubation. Incubation of macrophages with MIF-containing supernatants or partially purified MIF for 1-2 hr resulted in reduced cAMP accumulation in response to PGE1, PGE2, isoproterenol, and cholera toxin (nonspecific refractoriness). These findings indicate that MIF-induced inhibition of macrophage migration is not due to an increase in the cellular level of cAMP and that the reduction in cAMP concentration, caused by MIF, is probably a secondary phenomenon unrelated to the inhibition of cellular motility.
UR - http://www.scopus.com/inward/record.url?scp=0017706360&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(77)90209-X
DO - 10.1016/0008-8749(77)90209-X
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AN - SCOPUS:0017706360
SN - 0008-8749
VL - 32
SP - 329
EP - 339
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -
