TY - JOUR
T1 - The lpf operon of invasive Escherichia coli
AU - Ideses, Diana
AU - Biran, Dvora
AU - Gophna, Uri
AU - Levy-Nissenbaum, Orlev
AU - Ron, Eliora Z.
N1 - Funding Information:
The authors wish to thank Tslil Ophir for help with automated sequencing. D. Ideses thanks the Constantiner Institute for Molecular Genetics. U. Gophna is supported by a Killam postdoctoral fellowship. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.
PY - 2005/8/22
Y1 - 2005/8/22
N2 - Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.
AB - Extraintestinal pathogenic Escherichia coli (ExPEC) strains have been shown to code for several virulence factors involved in adherence to host tissues. Here we show the existence of an additional adherence gene cluster, coding for long polar fimbriae - LPF - in several strains of serotype O78 from septicemia and newborn meningitis. The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD. The lpf operon is expressed and is important for adherence to epithelial cells. The lpf operon was found only in four of the ExPEC strains tested and is likely to have been acquired by horizontal gene transfer.
KW - Adherence
KW - Avian colisepticemia
KW - Extraintestinal E. coli
KW - Newborn meningitis
KW - PhoA gene fusion
KW - Serotype O78
UR - http://www.scopus.com/inward/record.url?scp=22544446175&partnerID=8YFLogxK
U2 - 10.1016/j.ijmm.2005.04.009
DO - 10.1016/j.ijmm.2005.04.009
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AN - SCOPUS:22544446175
SN - 1438-4221
VL - 295
SP - 227
EP - 236
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology
IS - 4
ER -
