The Legionella pneumophila Sequencing Project

Xiaoyan Qu; Irina Morozova; Minchen Chen; Sergey Kalachikov; Gil Segal; Jing Chen; Hye Park; Anthi Georghiou; Gifty Asamani; Marc Feder; Justin Rineer; Joseph J. Greenberg; Curtis Goldsberry; Andrey Rzhetsky; Stuart G. Fischer; Pieter DeJong; Peisen Zhang; Eftihia Cayanis; Howard A. Shuman; James J. Russo

doi:10.1128/9781555817985.ch19

The Legionella pneumophila Sequencing Project

Xiaoyan Qu, Irina Morozova, Minchen Chen, Sergey Kalachikov, Gil Segal, Jing Chen, Hye Park, Anthi Georghiou, Gifty Asamani, Marc Feder, Justin Rineer, Joseph J. Greenberg, Curtis Goldsberry, Andrey Rzhetsky, Stuart G. Fischer, Pieter DeJong, Peisen Zhang, Eftihia Cayanis, Howard A. Shuman, James J. Russo

Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Summary This chapter aims at obtaining the complete sequence of the Philadelphia 1 strain of Legionella pneumophila, derived from the original 1976 outbreak that established the clinical entity known as Legionnaires' disease. The GC content of L. pneumophila is ~38more than 85 kb), 3,000 open reading frames (ORF) were covered, about 2,000 of which are complete, and on the basis of homology to genes from other prokaryotes, more than 1,100 putative genes in L. pneumophila were identified. Genes in L. pneumophila Philadelphia 1 can be compared with genes in other pathogenic organisms. The amino acid sequence can guide structural and functional analyses of enzymes and other proteins of the organism. The authors used knockout strategies in the past to assess the function of genes discovered in the course of the sequencing project; and this continues to be their preferred method for functional analysis.

Original language	English
Title of host publication	Legionella
Editors	Reinhard Marre, Yousef Abu Kwaik, Christophe Bartlett, Nicholas P. Cianciotto
Publisher	John Wiley & Sons, Ltd
Chapter	19
Pages	97-104
Number of pages	8
ISBN (Print)	9781683672357
DOIs	https://doi.org/10.1128/9781555817985.ch19
State	Published - 2001

Keywords

16S RNA homology
bacterial strain
clone sequencing
gene comparisons
gram-negative bacteria
intracellular life cycle
Legionella pneumophila
Legionnaires' disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/9781555817985.ch19

https://onlinelibrary.wiley.com/doi/abs/10.1128/9781555817985.ch19

Cite this

Qu, X., Morozova, I., Chen, M., Kalachikov, S., Segal, G., Chen, J., Park, H., Georghiou, A., Asamani, G., Feder, M., Rineer, J., Greenberg, J. J., Goldsberry, C., Rzhetsky, A., Fischer, S. G., DeJong, P., Zhang, P., Cayanis, E., Shuman, H. A., & Russo, J. J. (2001). The Legionella pneumophila Sequencing Project. In R. Marre, Y. Abu Kwaik, C. Bartlett, & N. P. Cianciotto (Eds.), Legionella (pp. 97-104). John Wiley & Sons, Ltd. https://doi.org/10.1128/9781555817985.ch19

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abstract = "Summary This chapter aims at obtaining the complete sequence of the Philadelphia 1 strain of Legionella pneumophila, derived from the original 1976 outbreak that established the clinical entity known as Legionnaires' disease. The GC content of L. pneumophila is ~38more than 85 kb), 3,000 open reading frames (ORF) were covered, about 2,000 of which are complete, and on the basis of homology to genes from other prokaryotes, more than 1,100 putative genes in L. pneumophila were identified. Genes in L. pneumophila Philadelphia 1 can be compared with genes in other pathogenic organisms. The amino acid sequence can guide structural and functional analyses of enzymes and other proteins of the organism. The authors used knockout strategies in the past to assess the function of genes discovered in the course of the sequencing project; and this continues to be their preferred method for functional analysis.",

keywords = "16S RNA homology, bacterial strain, clone sequencing, gene comparisons, gram-negative bacteria, intracellular life cycle, Legionella pneumophila, Legionnaires' disease",

author = "Xiaoyan Qu and Irina Morozova and Minchen Chen and Sergey Kalachikov and Gil Segal and Jing Chen and Hye Park and Anthi Georghiou and Gifty Asamani and Marc Feder and Justin Rineer and Greenberg, {Joseph J.} and Curtis Goldsberry and Andrey Rzhetsky and Fischer, {Stuart G.} and Pieter DeJong and Peisen Zhang and Eftihia Cayanis and Shuman, {Howard A.} and Russo, {James J.}",

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Qu, X, Morozova, I, Chen, M, Kalachikov, S, Segal, G, Chen, J, Park, H, Georghiou, A, Asamani, G, Feder, M, Rineer, J, Greenberg, JJ, Goldsberry, C, Rzhetsky, A, Fischer, SG, DeJong, P, Zhang, P, Cayanis, E, Shuman, HA & Russo, JJ 2001, The Legionella pneumophila Sequencing Project. in R Marre, Y Abu Kwaik, C Bartlett & NP Cianciotto (eds), Legionella. John Wiley & Sons, Ltd, pp. 97-104. https://doi.org/10.1128/9781555817985.ch19

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AU - Georghiou, Anthi

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AU - Goldsberry, Curtis

AU - Rzhetsky, Andrey

AU - Fischer, Stuart G.

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AB - Summary This chapter aims at obtaining the complete sequence of the Philadelphia 1 strain of Legionella pneumophila, derived from the original 1976 outbreak that established the clinical entity known as Legionnaires' disease. The GC content of L. pneumophila is ~38more than 85 kb), 3,000 open reading frames (ORF) were covered, about 2,000 of which are complete, and on the basis of homology to genes from other prokaryotes, more than 1,100 putative genes in L. pneumophila were identified. Genes in L. pneumophila Philadelphia 1 can be compared with genes in other pathogenic organisms. The amino acid sequence can guide structural and functional analyses of enzymes and other proteins of the organism. The authors used knockout strategies in the past to assess the function of genes discovered in the course of the sequencing project; and this continues to be their preferred method for functional analysis.

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KW - clone sequencing

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A2 - Bartlett, Christophe

A2 - Cianciotto, Nicholas P.

PB - John Wiley & Sons, Ltd

ER -

The Legionella pneumophila Sequencing Project

Abstract

Keywords

UN SDGs

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