TY - JOUR
T1 - The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model
AU - Livneh, Ayelet
AU - Youngster, Ilan
AU - El-On, Yossef
AU - Berkovitch, Matitiahu
AU - Abu-Kishk, Ibrahim
N1 - Publisher Copyright:
© 2015 W. Stefański Institute of Parasitology, PAS.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.
AB - We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.
KW - Leishmania major
KW - hyperbaric oxygen therapy
KW - methylbenzethonium chloride
KW - mouse model
KW - paromomycin sulphate
UR - http://www.scopus.com/inward/record.url?scp=84926306020&partnerID=8YFLogxK
U2 - 10.1515/ap-2015-0048
DO - 10.1515/ap-2015-0048
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C2 - 26204005
AN - SCOPUS:84926306020
SN - 1230-2821
VL - 60
SP - 345
EP - 349
JO - Acta Parasitologica
JF - Acta Parasitologica
IS - 2
ER -