The involvement of the sLe-a selectin ligand in the extravasation of human colorectal carcinoma cells

Tal Ben-David; Orit Sagi-Assif; Tsipi Meshel; Veronica Lifshitz; Ilana Yron; Isaac P. Witz

doi:10.1016/j.imlet.2007.11.022

The involvement of the sLe-a selectin ligand in the extravasation of human colorectal carcinoma cells

Tal Ben-David, Orit Sagi-Assif, Tsipi Meshel, Veronica Lifshitz, Ilana Yron, Isaac P. Witz^*

^*Corresponding author for this work

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The extravasation of tumor cells is a pivotal stage in the formation of hematogenous metastasis. An interaction of selectins expressed on endothelial cells and selectin ligands expressed by tumor cells has been implicated to play a role in extravasation. In the present study we used a human-mouse model to prove the hypothesis that the selectin ligand sialyl Lewis-a (sLe-a) is indeed involved in the in vivo extravasation of colorectal carcinoma (CRC) cells. The results indicated that highly metastatic CRC cells expressing high levels of sLe-a extravasate more efficiently than non-metastatic CRC cells expressing low levels of sLe-a. It was also demonstrated that down regulating the expression levels of sLe-a in CRC cells by genetic manipulations, significantly reduced CRC extravasation. Non-specific effects of these manipulations were ruled out. The results of this study indicate that the arrest and adhesion of CRC cells, and possibly of other types of cancer cells as well, to endothelium depend on the expression of the selectin ligand sLe-a by the tumor cells.

Original language	English
Pages (from-to)	218-224
Number of pages	7
Journal	Immunology Letters
Volume	116
Issue number	2
DOIs	https://doi.org/10.1016/j.imlet.2007.11.022
State	Published - 15 Mar 2008

Funding

Funders	Funder number
Arnold and Ruth Feuerstein
Ela Kodesz Institute for Research on Cancer Development and Prevention
Fainbarg Family Fund
Jacqueline Seroussi Memorial Foundation for Cancer Research
James J. Leibman and Rita S. Leibman Endowment Fund for Cancer Research
Natan Blutinger
Israel Cancer Association
Tel Aviv University
Ministry of Health, State of Israel

Keywords

Colorectal carcinoma
Experimental lung metastases
Extravasation
Fucosylated selectin ligands

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.imlet.2007.11.022

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title = "The involvement of the sLe-a selectin ligand in the extravasation of human colorectal carcinoma cells",

abstract = "The extravasation of tumor cells is a pivotal stage in the formation of hematogenous metastasis. An interaction of selectins expressed on endothelial cells and selectin ligands expressed by tumor cells has been implicated to play a role in extravasation. In the present study we used a human-mouse model to prove the hypothesis that the selectin ligand sialyl Lewis-a (sLe-a) is indeed involved in the in vivo extravasation of colorectal carcinoma (CRC) cells. The results indicated that highly metastatic CRC cells expressing high levels of sLe-a extravasate more efficiently than non-metastatic CRC cells expressing low levels of sLe-a. It was also demonstrated that down regulating the expression levels of sLe-a in CRC cells by genetic manipulations, significantly reduced CRC extravasation. Non-specific effects of these manipulations were ruled out. The results of this study indicate that the arrest and adhesion of CRC cells, and possibly of other types of cancer cells as well, to endothelium depend on the expression of the selectin ligand sLe-a by the tumor cells.",

keywords = "Colorectal carcinoma, Experimental lung metastases, Extravasation, Fucosylated selectin ligands",

author = "Tal Ben-David and Orit Sagi-Assif and Tsipi Meshel and Veronica Lifshitz and Ilana Yron and Witz, {Isaac P.}",

note = "Funding Information: This study was supported by: The Jacqueline Seroussi Memorial Foundation for Cancer Research; The Ela Kodesz Institute for Research on Cancer Development and Prevention, Tel Aviv University; The Israeli Ministry of Health; The Israel Cancer association, The Fainbarg Family Fund (Orange County, CA); The Fred August and Adele Wolpers Charitable Fund (Clifton, NJ); Bonnie and Steven Stern, (New York, NY); Arnold and Ruth Feuerstein (Orange County, CA); The Pikovsky Fund (Jerusalem, Israel); Natan Blutinger (West Orange, NJ); James J. Leibman and Rita S. Leibman Endowment Fund for Cancer Research.",

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T1 - The involvement of the sLe-a selectin ligand in the extravasation of human colorectal carcinoma cells

AU - Ben-David, Tal

AU - Sagi-Assif, Orit

AU - Meshel, Tsipi

AU - Lifshitz, Veronica

AU - Yron, Ilana

AU - Witz, Isaac P.

N1 - Funding Information: This study was supported by: The Jacqueline Seroussi Memorial Foundation for Cancer Research; The Ela Kodesz Institute for Research on Cancer Development and Prevention, Tel Aviv University; The Israeli Ministry of Health; The Israel Cancer association, The Fainbarg Family Fund (Orange County, CA); The Fred August and Adele Wolpers Charitable Fund (Clifton, NJ); Bonnie and Steven Stern, (New York, NY); Arnold and Ruth Feuerstein (Orange County, CA); The Pikovsky Fund (Jerusalem, Israel); Natan Blutinger (West Orange, NJ); James J. Leibman and Rita S. Leibman Endowment Fund for Cancer Research.

PY - 2008/3/15

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N2 - The extravasation of tumor cells is a pivotal stage in the formation of hematogenous metastasis. An interaction of selectins expressed on endothelial cells and selectin ligands expressed by tumor cells has been implicated to play a role in extravasation. In the present study we used a human-mouse model to prove the hypothesis that the selectin ligand sialyl Lewis-a (sLe-a) is indeed involved in the in vivo extravasation of colorectal carcinoma (CRC) cells. The results indicated that highly metastatic CRC cells expressing high levels of sLe-a extravasate more efficiently than non-metastatic CRC cells expressing low levels of sLe-a. It was also demonstrated that down regulating the expression levels of sLe-a in CRC cells by genetic manipulations, significantly reduced CRC extravasation. Non-specific effects of these manipulations were ruled out. The results of this study indicate that the arrest and adhesion of CRC cells, and possibly of other types of cancer cells as well, to endothelium depend on the expression of the selectin ligand sLe-a by the tumor cells.

AB - The extravasation of tumor cells is a pivotal stage in the formation of hematogenous metastasis. An interaction of selectins expressed on endothelial cells and selectin ligands expressed by tumor cells has been implicated to play a role in extravasation. In the present study we used a human-mouse model to prove the hypothesis that the selectin ligand sialyl Lewis-a (sLe-a) is indeed involved in the in vivo extravasation of colorectal carcinoma (CRC) cells. The results indicated that highly metastatic CRC cells expressing high levels of sLe-a extravasate more efficiently than non-metastatic CRC cells expressing low levels of sLe-a. It was also demonstrated that down regulating the expression levels of sLe-a in CRC cells by genetic manipulations, significantly reduced CRC extravasation. Non-specific effects of these manipulations were ruled out. The results of this study indicate that the arrest and adhesion of CRC cells, and possibly of other types of cancer cells as well, to endothelium depend on the expression of the selectin ligand sLe-a by the tumor cells.

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KW - Experimental lung metastases

KW - Extravasation

KW - Fucosylated selectin ligands

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The involvement of the sLe-a selectin ligand in the extravasation of human colorectal carcinoma cells

Abstract

Funding

Keywords

UN SDGs

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