The involvement of superoxide radicals in medium pressure UV derived inactivation

Yoram Gerchman, Vered Cohen-Yaniv, Yifaat Betzalel, Sharon Yagur-Kroll, Shimshon Belkin, Hadas Mamane

Research output: Contribution to journalArticlepeer-review

Abstract

Today, two types of lamp systems dominate the UV disinfection industry: low-pressure (LP) UV lamps and medium-pressure (MP) polychromatic lamps. Both lamp types have their advantages and disadvantages in microorganism inactivation, with LP lamps being cheaper, having longer life, and working at lower temperature, hence reducing fouling, and MP lamps showing better inactivation per germicidal dose for certain microorganisms. Bacterium-based biosensors were used to compare LP and MP irradiation. These biosensors were Escherichia coli bacteria carrying the lux operon genes under the control of different stress-responding promoters, where activation of the specific promoter is manifested as bioluminescence. MP irradiation, considerably more than LP irradiation, resulted in activation of the superoxide dismutase expression, indicating the formation of superoxide radicals inside the cells. Accordingly, pre-exposure (immunization) of the bacteria to an activator that produces superoxide radicals resulted in lower inactivation and increased resistance to MP irradiation, but not to LP irradiation. This study shows that the difference in germicidal efficiency may result from the production of intracellular superoxide radicals by MP irradiation, at wavelengths other than 254 nm, as emitted by LP lamps.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalWater Research
Volume161
DOIs
StatePublished - 15 Sep 2019

Keywords

  • Bacteria
  • Bioreporter
  • Mechanism
  • Superoxide radical
  • UV disinfection

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