The interaction of heme-hemopexin with CO

N. Shaklai, V. S. Sharma, U. Muller-Eberhard, W. T. Morgan

Research output: Contribution to journalArticlepeer-review


The equilibria and kinetics of the reaction of heme-hemopexin with CO were studied. A stoichiometry of one CO/heme was determined, and the affinity of heme-hemopexin for CO was found to be pH-dependent. At pH 8.0, the affinity constant was 4.5x105 M-1 compared with 4x106 M-1 at pH 6.1. The kinetics of CO binding were also pH-dependent. A biphasic reaction at neutral pH could be resolved into a faster phase (k(on)=2.2x103 M-1s-1) solely found at pH 6.0, and a slower phase (k(on)=2.0x102 M-1s-1) solely found at pH 8.0. The dissociation reaction on the other hand was found to be independent of pH in the range examined (k(off)=5x10-4s-1).

Original languageEnglish
Pages (from-to)1544-1548
Number of pages5
JournalJournal of Biological Chemistry
Issue number4
StatePublished - 1981


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