The interaction of brain insulin receptors with wheat germ agglutinin

Joshua Shemer, Derek LeRoith

Research output: Contribution to journalArticlepeer-review

Abstract

Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photoaffinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalNeuropeptides
Volume9
Issue number1
DOIs
StatePublished - Jan 1987
Externally publishedYes

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