The initial phase of GnRH-stimulated LH release from pituitary cells is independent of calcium entry through voltage-gated channels

Colleen E. Smith*, I. Wakefield, Judy A. King, Z. Naor, R. P. Millar, J. S. Davidson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Kinetic studies on gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone (LH) release were undertaken using rat and chicken pituitary cell cultures. In response to continuous GnRH stimulation, a biphasic pattern of LH release was demonstrated. The two phases showed different susceptibility to the voltage-gated Ca2+ channel blockers D600 and nifedipine. The first (transient) phase of LH release was unaffected by the Ca2+ channel blockers whereas the second (sustained) phase was inhibited by both drugs. These results indicate that the initial phase of LH release is independent of Ca2+ entry through voltage-gated Ca2+ channels and may depend on mobilisation of intracellular Ca2+ or entry of extracellular Ca2+ through another mechanism.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume225
Issue number1-2
DOIs
StatePublished - 10 Dec 1987
Externally publishedYes

Funding

FundersFunder number
South African Medical ResearchC ouncil

    Keywords

    • (Pituitary cell)
    • Ca channel
    • Ca influx
    • Gonadotropin-releasing hormone
    • Hormone release
    • Kinetics
    • Luteinizing hormone

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