TY - JOUR
T1 - The influence of in vivo exposure to nonylphenol ethoxylate 10 (NP-10) on the ovarian reserve in a mouse model
AU - Shufaro, Yoel
AU - Saada, Ann
AU - Simeonov, Monica
AU - Tsuberi, Ben Zion
AU - Alban, Corinne
AU - Kogot-Levin, Aviram
AU - Shochat, Tzippy
AU - Fisch, Benjamin
AU - Abir, Ronit
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/10
Y1 - 2018/10
N2 - Aim: To determine the effect of nonylphenol-ethoxylate-10 (NP-10) on the ovarian reserve in a mouse model. Design: Female mice were maintained on purified water or exposed to NP-10 from 3-7-weeks of age. At 7-weeks they were stimulated, mated and the zygotes were cultured in-vitro. Three and 7-weeks old mice were untreated controls. Identical groups were sacrificed without stimulation. Ovaries were analysed for follicular composition. Respiratory-chain (RC) activity and reactive-oxygen-species (ROS) production were measured in brains and livers. Results: Seven-weeks-old mice produced fewer oocytes/embryos than 3-week-old mice. At 7-weeks, mice exposed to NP-10 produced more oocytes/embryos the controls. Their ovaries contained more primordial/primary follicles, with a lower rate of proliferation and fewer antral follicles. There were no differences in follicular apoptosis, RC-activity or ROS production. Conclusions: In this model, exposure to NP-10 inhibited the spontaneous follicular recruitment, the first report of successful inhibition of physiologic ovarian aging, to the best of our knowledge.
AB - Aim: To determine the effect of nonylphenol-ethoxylate-10 (NP-10) on the ovarian reserve in a mouse model. Design: Female mice were maintained on purified water or exposed to NP-10 from 3-7-weeks of age. At 7-weeks they were stimulated, mated and the zygotes were cultured in-vitro. Three and 7-weeks old mice were untreated controls. Identical groups were sacrificed without stimulation. Ovaries were analysed for follicular composition. Respiratory-chain (RC) activity and reactive-oxygen-species (ROS) production were measured in brains and livers. Results: Seven-weeks-old mice produced fewer oocytes/embryos than 3-week-old mice. At 7-weeks, mice exposed to NP-10 produced more oocytes/embryos the controls. Their ovaries contained more primordial/primary follicles, with a lower rate of proliferation and fewer antral follicles. There were no differences in follicular apoptosis, RC-activity or ROS production. Conclusions: In this model, exposure to NP-10 inhibited the spontaneous follicular recruitment, the first report of successful inhibition of physiologic ovarian aging, to the best of our knowledge.
KW - Ageing
KW - Mitochondria
KW - Nonylphenol ethoxylate 10 (NP-10 Tergitol)
KW - Ovarian reserve
KW - Primordial follicle pool
UR - http://www.scopus.com/inward/record.url?scp=85052875263&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2018.08.020
DO - 10.1016/j.reprotox.2018.08.020
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AN - SCOPUS:85052875263
SN - 0890-6238
VL - 81
SP - 246
EP - 252
JO - Reproductive Toxicology
JF - Reproductive Toxicology
ER -