The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism

A. Klein; H. Kaufmann; R. Arie; H. G.D. Hoogervorst-Spalter; H. Joshua

doi:10.1007/BF01917912

The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism

A. Klein^*, H. Kaufmann, R. Arie, H. G.D. Hoogervorst-Spalter, H. Joshua

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The influence of a) tissue culture medium (RPMI), b) homologous plasma (HP), and c) fetal calf serum (FCS) on lymphocytic cortisol metabolism was compared to that of phosphate buffered saline alone. RPMI was found to enhance the conversion rate 1.71 times, whereas HP and FCS enhanced it about 3.2 times. Raising the temperature of the HP and FCS to 100°C before incubation reduced the enhancing effect to the level of that obtained with RPMI.

Original language	English
Pages (from-to)	114-115
Number of pages	2
Journal	Experientia
Volume	35
Issue number	1
DOIs	https://doi.org/10.1007/BF01917912
State	Published - Jan 1979
Externally published	Yes

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abstract = "The influence of a) tissue culture medium (RPMI), b) homologous plasma (HP), and c) fetal calf serum (FCS) on lymphocytic cortisol metabolism was compared to that of phosphate buffered saline alone. RPMI was found to enhance the conversion rate 1.71 times, whereas HP and FCS enhanced it about 3.2 times. Raising the temperature of the HP and FCS to 100°C before incubation reduced the enhancing effect to the level of that obtained with RPMI.",

author = "A. Klein and H. Kaufmann and R. Arie and Hoogervorst-Spalter, {H. G.D.} and H. Joshua",

year = "1979",

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AU - Joshua, H.

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AB - The influence of a) tissue culture medium (RPMI), b) homologous plasma (HP), and c) fetal calf serum (FCS) on lymphocytic cortisol metabolism was compared to that of phosphate buffered saline alone. RPMI was found to enhance the conversion rate 1.71 times, whereas HP and FCS enhanced it about 3.2 times. Raising the temperature of the HP and FCS to 100°C before incubation reduced the enhancing effect to the level of that obtained with RPMI.

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The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism

Abstract

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