The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase

Yoram Wollman; Shmariahu Blumberg; Anya Spungin; Douglas E. Brenneman; Mati Fridkin; Jonathan Wollman; Adrian Iaina; Illana Gozes

doi:10.1016/S0167-0115(02)00098-8

The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase

Yoram Wollman, Shmariahu Blumberg, Anya Spungin, Douglas E. Brenneman, Mati Fridkin, Jonathan Wollman, Adrian Iaina, Illana Gozes^*

^*Corresponding author for this work

Human Molecular Genetics Biochemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) stimulates the neuroblastoma cell line (NMB) to proliferate. Neuropeptide activity can be inhibited by neutral endopeptidases that function intracellularly and in the extracellular milieu. NMB cells express neutral endopeptidase (NEP) activity that can be specifically inhibited by phosphoramidon (PA). Our data now show that phosphoramidon treatment increases the efficacy of VIP-stimulated neuroblastoma proliferation. These results suggest that membrane endopeptidases modulate VIP-associated cell proliferation and enhancement of endopeptidase activity may serve as a target for cancer therapy.

Original language	English
Pages (from-to)	175-177
Number of pages	3
Journal	Regulatory Peptides
Volume	108
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-0115(02)00098-8
State	Published - 15 Oct 2002

Funding

Funders	Funder number
ISOA
Bloom's Syndrome Foundation
Iowa Science Foundation

Keywords

Neuroblastoma cells
Neutral endopeptidase
Vasoactive intestinal peptide

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0167-0115(02)00098-8

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title = "The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase",

abstract = "Vasoactive intestinal peptide (VIP) stimulates the neuroblastoma cell line (NMB) to proliferate. Neuropeptide activity can be inhibited by neutral endopeptidases that function intracellularly and in the extracellular milieu. NMB cells express neutral endopeptidase (NEP) activity that can be specifically inhibited by phosphoramidon (PA). Our data now show that phosphoramidon treatment increases the efficacy of VIP-stimulated neuroblastoma proliferation. These results suggest that membrane endopeptidases modulate VIP-associated cell proliferation and enhancement of endopeptidase activity may serve as a target for cancer therapy.",

keywords = "Neuroblastoma cells, Neutral endopeptidase, Vasoactive intestinal peptide",

author = "Yoram Wollman and Shmariahu Blumberg and Anya Spungin and Brenneman, {Douglas E.} and Mati Fridkin and Jonathan Wollman and Adrian Iaina and Illana Gozes",

note = "Funding Information: This paper is in memory of our dear colleague and friend Professor Shmariahu Blumberg. Supported by BSF, ISOA, Neufeld award, ISF and Lily and Avraham Gildor Chair for the investigation of growth factors. We thank Dr. Avron D. Spier for the critical reading of this manuscript.",

year = "2002",

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doi = "10.1016/S0167-0115(02)00098-8",

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Wollman, Y, Blumberg, S, Spungin, A, Brenneman, DE, Fridkin, M, Wollman, J, Iaina, A & Gozes, I 2002, 'The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase', Regulatory Peptides, vol. 108, no. 2-3, pp. 175-177. https://doi.org/10.1016/S0167-0115(02)00098-8

TY - JOUR

T1 - The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase

AU - Wollman, Yoram

AU - Blumberg, Shmariahu

AU - Spungin, Anya

AU - Brenneman, Douglas E.

AU - Fridkin, Mati

AU - Wollman, Jonathan

AU - Iaina, Adrian

AU - Gozes, Illana

N1 - Funding Information: This paper is in memory of our dear colleague and friend Professor Shmariahu Blumberg. Supported by BSF, ISOA, Neufeld award, ISF and Lily and Avraham Gildor Chair for the investigation of growth factors. We thank Dr. Avron D. Spier for the critical reading of this manuscript.

PY - 2002/10/15

Y1 - 2002/10/15

N2 - Vasoactive intestinal peptide (VIP) stimulates the neuroblastoma cell line (NMB) to proliferate. Neuropeptide activity can be inhibited by neutral endopeptidases that function intracellularly and in the extracellular milieu. NMB cells express neutral endopeptidase (NEP) activity that can be specifically inhibited by phosphoramidon (PA). Our data now show that phosphoramidon treatment increases the efficacy of VIP-stimulated neuroblastoma proliferation. These results suggest that membrane endopeptidases modulate VIP-associated cell proliferation and enhancement of endopeptidase activity may serve as a target for cancer therapy.

AB - Vasoactive intestinal peptide (VIP) stimulates the neuroblastoma cell line (NMB) to proliferate. Neuropeptide activity can be inhibited by neutral endopeptidases that function intracellularly and in the extracellular milieu. NMB cells express neutral endopeptidase (NEP) activity that can be specifically inhibited by phosphoramidon (PA). Our data now show that phosphoramidon treatment increases the efficacy of VIP-stimulated neuroblastoma proliferation. These results suggest that membrane endopeptidases modulate VIP-associated cell proliferation and enhancement of endopeptidase activity may serve as a target for cancer therapy.

KW - Neuroblastoma cells

KW - Neutral endopeptidase

KW - Vasoactive intestinal peptide

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SN - 0167-0115

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ER -

The increased proliferation of cultured neuroblastoma cells treated with vasoactive intestinal peptide is enhanced by simultaneous inhibition of neutral endopeptidase

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