The identification of secreted heat shock 60 -like protein from rat glial cells and a human neuroblastoma cell line

Merav Bassan, Rachel Zamostiano, Eliezer Giladi, Ariane Davidson, Yoram Wollman, Jessica Pitman, Janet Hauser, Douglas E. Brenneman, Illana Gozes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The intracellular stress-induced proteins provide protection against toxic insults. Here, a 60 000-Da heat shock 60 (hsp60)-like protein was detected, with five different antibodies, in conditioned media derived from rat cortical astrocytes and a human neuroblastoma cell line. Extracellular neuroblastoma hsp60-like immunoreactivity was increased 3-fold in the presence of the neuropeptide vasoactive intestinal peptide (VIP) and was augmented 2-fold after temperature elevation. Intracellular hsp60 immunoreactivity was reduced 2-3-fold in the presence of VIP; this reduction was attenuated in the presence of brefeldin A, an inhibitor of protein secretion. In contrast, the activity of lactate dehydrogenase (LDH), an intracellular marker, did not change in the presence of VIP. Essentially no extracellular LDH activity was detected, indicating no cellular damage. A novel aspect for stress proteins having extracellular protective roles is suggested.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalNeuroscience Letters
Volume250
Issue number1
DOIs
StatePublished - 26 Jun 1998

Keywords

  • Astrocytes
  • Heat shock-like 60 protein
  • Neuroblastoma
  • Stress proteins
  • Vasoactive intestinal peptide

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