The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae

Ian Dix, Caroline Russell, Sigal Ben Yehuda, Martin Kupiec, Jean D. Beggs

Research output: Contribution to journalArticlepeer-review

Abstract

We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1p is important in the optimization of splicing.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalRNA
Volume5
Issue number3
DOIs
StatePublished - Mar 1999

Keywords

  • Spliceosome
  • Yeast
  • pre-mRNA splicing
  • snRNA
  • snRNPs

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