TY - JOUR
T1 - The human surfeit locus
AU - Duhig, Trevor
AU - Ruhrberg, Christiana
AU - Mor, Orna
AU - Fried, Mike
N1 - Funding Information:
blotting was performed using the standard protocols suggested in the Hybond-N protocol booklet from Amersham, and Hybond-N membrane was used in all cases. All DNA probes were radiolabeled by random hexanucleotide priming and hybridized to membranes under standard conditions. The human chromosome 9-specific gene library LL09NC01 used in this work was constructed at the Biomedical Sciences Division, Lawrence Livermore National Laboratory (Livermore, CA) under the auspices of the National Laboratory Gene Library Project sponsored by the U.S. Department of Energy. The Human P1 Library No. 700 and the Human PAC Library No. 704 were obtained from the Reference Library Database at the Max-Planck-Institute for Molecular Genetics (Berlin–Dahlem, Germany). A random-primed human fetal brain Lambda ZAP II cDNA library was obtained from Stratagene Ltd., Cambridge. High-stringency hybridizations to the libraries and Southern blots were performed at 65°C, and washes were performed at 65°C in 0.1 × SSC (1 × SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 0.1% sodium dodecyl sulfate (SDS).
PY - 1998/8/15
Y1 - 1998/8/15
N2 - The organization of the human Surfeit locus containing the six sequence- unrelated housekeeping genes Surf-1 to Surf-6 (HGMW-approved symbols SURF1- SURF6) has been determined. The human surfeit locus occupies about 60 kb of DNA, and the tightly clustered gene organization and the juxtaposition of the human genes are similar to the mouse and chicken surfeit loci with the 5' end of each gene associated with a CpG-rich island. Whereas in the mouse the Surf-2 and Surf-4 genes overlap at their 3' ends, the human Surf-2 and Surf- 4 genes have been found to be separated by 302 bp due to a much shorter 3' untranslated region in the human Surf-2 gene. The distance between the 3' ends of the human Surf-1 and Surf-3 genes is 374 bp, and the distance between the 5' ends of the human Surf-3 and Surf-5 genes is only 112 bp. Unusually the human Surf-5 gene contains an intron in its 5' untranslated region not found in the mouse or rat Surf-5 genes. This additional intron is also found in the Surf-5 gene of both Old and New World monkeys, being generated before the divergence of human and prosimians but after the divergence of primates and rodents. A contig of 200 kb containing the human Surfeit locus has been constructed from overlapping cosmid, P1, and PAC clones. Approximately 40 kb proximal to the 3' end of the Surf-6 gene, the 5' region of the ABO glycosyltransferase gene has been detected. This allows us to determine the orientation of the Surfeit and ABO loci with respect to each other and to the telomere and centromere of human chromosome 9.
AB - The organization of the human Surfeit locus containing the six sequence- unrelated housekeeping genes Surf-1 to Surf-6 (HGMW-approved symbols SURF1- SURF6) has been determined. The human surfeit locus occupies about 60 kb of DNA, and the tightly clustered gene organization and the juxtaposition of the human genes are similar to the mouse and chicken surfeit loci with the 5' end of each gene associated with a CpG-rich island. Whereas in the mouse the Surf-2 and Surf-4 genes overlap at their 3' ends, the human Surf-2 and Surf- 4 genes have been found to be separated by 302 bp due to a much shorter 3' untranslated region in the human Surf-2 gene. The distance between the 3' ends of the human Surf-1 and Surf-3 genes is 374 bp, and the distance between the 5' ends of the human Surf-3 and Surf-5 genes is only 112 bp. Unusually the human Surf-5 gene contains an intron in its 5' untranslated region not found in the mouse or rat Surf-5 genes. This additional intron is also found in the Surf-5 gene of both Old and New World monkeys, being generated before the divergence of human and prosimians but after the divergence of primates and rodents. A contig of 200 kb containing the human Surfeit locus has been constructed from overlapping cosmid, P1, and PAC clones. Approximately 40 kb proximal to the 3' end of the Surf-6 gene, the 5' region of the ABO glycosyltransferase gene has been detected. This allows us to determine the orientation of the Surfeit and ABO loci with respect to each other and to the telomere and centromere of human chromosome 9.
UR - http://www.scopus.com/inward/record.url?scp=0032528945&partnerID=8YFLogxK
U2 - 10.1006/geno.1998.5372
DO - 10.1006/geno.1998.5372
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 9740673
AN - SCOPUS:0032528945
SN - 0888-7543
VL - 52
SP - 72
EP - 78
JO - Genomics
JF - Genomics
IS - 1
ER -