The host DNA sequences in different populations of serially passaged SV40

Niza Frenkel*, Sara Lavi, Ernest Winocour

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The closed-circular SV40 DNA which evolved during 4 independent sets of high-multiplicity serial passages, initiated from plaque-purified virus, was studied by reassociation kinetics. The results indicate that the host DNA sequences covalently linked to viral sequences in such serially passaged SV40 DNA, (1) are predominantly of the nonreiterated type, (2) are similar in populations which evolved during a given set of serial passages, (3) can be different in populations which arose during different sets of serial passages starting from the same plaque isolate, and (4) do not represent a random selection of the total sequences present in the host cell genome. These results are discussed in terms of the viral recombination sites on the host genome and the consequent evolution of virus DNA molecules containing host DNA sequences.

Original languageEnglish
Pages (from-to)9-20
Number of pages12
JournalVirology
Volume60
Issue number1
DOIs
StatePublished - Jul 1974
Externally publishedYes

Funding

FundersFunder number
National Cancer Institute
U.S. Public Health ServiceCA-14530f

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