The herpes simplex virus amplicon IV. Efficient expression of a chimeric chicken ovalbumin gene amplified within defective virus genomes

Ann D. Kwong, Niza Frenkel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

cDNA sequences of the chicken ovalbumin gene were fused to an α (immediate early) promoter of herpes simplex virus and to genomic ovalbumin 3′-flanking sequences. The chimeric α-ovalbumin gene was introduced into defective virus genomes which were stably propagated in serially passaged virus stocks in the presence of helper virus. Analyses of polypeptides synthesized in cells infected with the resultant defective virus stocks revealed the abundant expression of the chimeric α-ovalbumin gene. The presence of introns was not essential for this expression.

Original languageEnglish
Pages (from-to)421-425
Number of pages5
JournalVirology
Volume142
Issue number2
DOIs
StatePublished - 30 Apr 1985
Externally publishedYes

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