The herpes simplex virus amplicon: Analyses of cis-acting replication functions

R. R. Spaete, N. Frenkel

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that two separate cis-acting functions - a DNA replication origin and a cleavage/packaging signal - are required for amplicon propagation. Using deleted derivatives of cloned amplicons, we mapped one of the viral DNA replication origins (ori-2 or ori(L)) at coordinate 0.422 of the standard HSV-1 genome and at an equivalent position within the HSV-2 genome.

Original languageEnglish
Pages (from-to)694-698
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number3
DOIs
StatePublished - 1985
Externally publishedYes

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