TY - JOUR
T1 - The Gut Microbiome of Adults With Type 1 Diabetes and Its Association With the Host Glycemic Control
AU - Shilo, Smadar
AU - Godneva, Anastasia
AU - Rachmiel, Marianna
AU - Korem, Tal
AU - Bussi, Yuval
AU - Kolobkov, Dmitry
AU - Karady, Tal
AU - Bar, Noam
AU - Wolf, Bat Chen
AU - Glantz-Gashai, Yitav
AU - Cohen, Michal
AU - Levin, Nehama Zuckerman
AU - Shehadeh, Naim
AU - Gruber, Noah
AU - Levran, Neriya
AU - Koren, Shlomit
AU - Weinberger, Adina
AU - Pinhas-Hamiel, Orit
AU - Segal, Eran
N1 - Publisher Copyright:
© 2022 by the American Diabetes Association.
PY - 2022/3
Y1 - 2022/3
N2 - OBJECTIVE Previous studies have demonstrated an association between gut microbiota composition and type 1 diabetes (T1D) pathogenesis. However, little is known about the composition and function of the gut microbiome in adults with longstanding T1D or its association with host glycemic control. RESEARCH DESIGN AND METHODS We performed a metagenomic analysis of the gut microbiome obtained from fecal samples of 74 adults with T1D, 14.6 ± 9.6 years following diagnosis, and compared their microbial composition and function to 296 age-matched healthy control subjects (1:4 ratio). We further analyzed the association between microbial taxa and indices of glycemic control derived from continuous glucose monitoring measure-ments and blood tests and constructed a prediction model that solely takes micro-biome features as input to evaluate the discriminative power of microbial composition for distinguishing individuals with T1D from control subjects. RESULTS Adults with T1D had a distinct microbial signature that separated them from control subjects when using prediction algorithms on held-out subjects (area under the receiver operating characteristic curve 5 0.89 ± 0.03). Linear discriminant analysis showed several bacterial species with significantly higher scores in T1D, including Prevotella copri and Eubacterium siraeum, and species with higher scores in control subjects, including Firmicutes bacterium and Faecalibacterium prausnitzii (P < 0.05, false discovery rate corrected for all). On the functional level, several metabolic pathways were significantly lower in adults with T1D. Several bacterial taxa and metabolic pathways were associated with the host’s glycemic control. CONCLUSIONS We identified a distinct gut microbial signature in adults with longstanding T1D and associations between microbial taxa, metabolic pathways, and glycemic control indices. Additional mechanistic studies are needed to identify the role of these bacteria for potential therapeutic strategies.
AB - OBJECTIVE Previous studies have demonstrated an association between gut microbiota composition and type 1 diabetes (T1D) pathogenesis. However, little is known about the composition and function of the gut microbiome in adults with longstanding T1D or its association with host glycemic control. RESEARCH DESIGN AND METHODS We performed a metagenomic analysis of the gut microbiome obtained from fecal samples of 74 adults with T1D, 14.6 ± 9.6 years following diagnosis, and compared their microbial composition and function to 296 age-matched healthy control subjects (1:4 ratio). We further analyzed the association between microbial taxa and indices of glycemic control derived from continuous glucose monitoring measure-ments and blood tests and constructed a prediction model that solely takes micro-biome features as input to evaluate the discriminative power of microbial composition for distinguishing individuals with T1D from control subjects. RESULTS Adults with T1D had a distinct microbial signature that separated them from control subjects when using prediction algorithms on held-out subjects (area under the receiver operating characteristic curve 5 0.89 ± 0.03). Linear discriminant analysis showed several bacterial species with significantly higher scores in T1D, including Prevotella copri and Eubacterium siraeum, and species with higher scores in control subjects, including Firmicutes bacterium and Faecalibacterium prausnitzii (P < 0.05, false discovery rate corrected for all). On the functional level, several metabolic pathways were significantly lower in adults with T1D. Several bacterial taxa and metabolic pathways were associated with the host’s glycemic control. CONCLUSIONS We identified a distinct gut microbial signature in adults with longstanding T1D and associations between microbial taxa, metabolic pathways, and glycemic control indices. Additional mechanistic studies are needed to identify the role of these bacteria for potential therapeutic strategies.
UR - http://www.scopus.com/inward/record.url?scp=85125882567&partnerID=8YFLogxK
U2 - 10.2337/dc21-1656
DO - 10.2337/dc21-1656
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 35045174
AN - SCOPUS:85125882567
SN - 0149-5992
VL - 45
SP - 555
EP - 563
JO - Diabetes Care
JF - Diabetes Care
IS - 3
ER -
