TY - JOUR
T1 - The expression of mannose-ligand receptor is correlated with sperm morphology
AU - Gamzu, R.
AU - Yogev, L.
AU - Amnon, B.
AU - Kleiman, S.
AU - Hauser, R.
AU - Lessing, J. B.
AU - Paz, G.
AU - Yavetz, H.
PY - 2002/11
Y1 - 2002/11
N2 - The objective of the present study was to evaluate the association between the expression of sperm mannose-ligand receptors and sperm morphology. Sperm samples were obtained from 45 men, 30 fertile sperm donors and 15 infertile men. Sperm concentration, motility and morphology were evaluated and then incubated with control medium (Ham's F-10 + 1% HSA) for 4 h. Expression of mannose-ligand receptors was evaluated by mannosylated-BSA-FITC (subdivided into 3 patterns: 1, for uncapacitated sperm; II, for capacitated; and III, for acrosome-reacted sperm). The mean (± SE) frequencies of sperm cells of the total sperm population that expressed patterns 1, II, and III were 88 ± 2.1% 7 ± 1.6%, and 5 ± 0.8%, respectively, for fertile men, and 90 ± 2.1%, 7 ± 1.3%, and 3 ± 0.5%, respectively, for infertile men. The rate of pattern III expression of mannose-ligand receptors was significantly higher in the fertile group compared to the infertile patients (p < .01). A poor but significant correlation was observed between the rate of pattern III and the percentage of normal-forms sperm cell in the ejaculate (r = .35, p - .018). Fertile sperm samples express more advanced patterns of mannose-ligand receptors compared to infertile men. This phenomenon is related to the morphology of human sperm cell in the ejaculate more than to any other basic sperm characteristics.
AB - The objective of the present study was to evaluate the association between the expression of sperm mannose-ligand receptors and sperm morphology. Sperm samples were obtained from 45 men, 30 fertile sperm donors and 15 infertile men. Sperm concentration, motility and morphology were evaluated and then incubated with control medium (Ham's F-10 + 1% HSA) for 4 h. Expression of mannose-ligand receptors was evaluated by mannosylated-BSA-FITC (subdivided into 3 patterns: 1, for uncapacitated sperm; II, for capacitated; and III, for acrosome-reacted sperm). The mean (± SE) frequencies of sperm cells of the total sperm population that expressed patterns 1, II, and III were 88 ± 2.1% 7 ± 1.6%, and 5 ± 0.8%, respectively, for fertile men, and 90 ± 2.1%, 7 ± 1.3%, and 3 ± 0.5%, respectively, for infertile men. The rate of pattern III expression of mannose-ligand receptors was significantly higher in the fertile group compared to the infertile patients (p < .01). A poor but significant correlation was observed between the rate of pattern III and the percentage of normal-forms sperm cell in the ejaculate (r = .35, p - .018). Fertile sperm samples express more advanced patterns of mannose-ligand receptors compared to infertile men. This phenomenon is related to the morphology of human sperm cell in the ejaculate more than to any other basic sperm characteristics.
KW - Mannose-ligand receptors
KW - Morphology
KW - Sperm
UR - http://www.scopus.com/inward/record.url?scp=79961197419&partnerID=8YFLogxK
U2 - 10.1080/01485010290099273
DO - 10.1080/01485010290099273
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C2 - 12425765
AN - SCOPUS:79961197419
SN - 0148-5016
VL - 48
SP - 475
EP - 480
JO - Archives of Andrology
JF - Archives of Andrology
IS - 6
ER -